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Reporter gene fusion type not specified. |
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Expr1327
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Expression of the lir-1::lacZ construct pML169, which should reflect the expression of all lir-1 isoforms, and of the lir-1::lacZ construct pML165, which should reflect only the expression of lir-1 isoforms starting after the second exon, were first very weakly detected at the 200-cell stage and then clearly detected at the 350-cell stage in major hypodermal cells and at the early comma stage in those same hypodermal cells, as well as in support cells and in all minor hypodermal cells. Starting at the late comma stage, expression of the lir-1::lacZ construct pML169, but not that of pML165, was detected in all cells (i.e., in intestinal, pharyngeal, muscle, neuronal, hypodermal, and support cells). Similarly, during larval development, expression of the lir-1::lacZ pML169 was detected in virtually all cells, including cells of the somatic gonad, but not in intestinal cells after the late L1 stage nor in germ cells. |
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Expr16046
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GFP was first observed in the seam cells of hypodermis in late embryos. The expression continues through the larval stages as exemplified by the fluorescence seen in the posterior part of an L3 larva. Strong intestinal expression was visible from early larval to adult stages. Expression in neurons was detected in larvae and adults. Bright expression was visible in canal-associated (CAN) neurons after the L4 molt. Expression in somatic gonad cells, such as spermatheca and sheath cells, were detected in adults. |
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For in situ hybridization, the signal from anti-sense probes was abolished by ribonuclease treatment of embryos. cDNA clone used as template for unidirectional PCR began at position 14 and ended at 2033 to remove the poly-A tract. For reporter gene, integrant gave same results, confirming that mosaicism. was not responsible for pattern. Reporter gene results gave resolution of cells. Transgenic Marker: rol-6(su1006). Reporter gene and in situ hybridization gave similar results, but lacZ expression was more intense in gut than pharynx. Hybridization results differed in being more intense in pharynx. early embryo(author) = late cleavage stage embryo(curator). late embryo(author) = comma + 1.5-fold + 2-fold + 3-fold + fully-elongated embryo(curator). |
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Expr509
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In the gut at the 8E cell stage; E descendants continue to show expression through late embryo. Pharyngeal cells derived from both AB and MS lineages show staining starting at comma stage. Intestinal and pharyngeal staining decline after hatching. The developing somatic gonad shows expression at L3 and L4. In comma to 1.5-fold stage embryos, hybridization was also seen in the tail region. |
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Data observed from, atIs13, atEx32 and atEx35. |
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Expr970
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In comma to 1.5-fold stage embryos, the nhr-25::GFP are expressed in the V cells, P cells and hyp7. Expression also observed in the head and tail hypodermal cells of embryos. The earliest expression is at ~250-300 min post fertilization. atEx32 and atEx35 L1 animals exhibited consistent reporter expression in the hyp7 and P cell nuclei. The P cell expression was very strong at hatching. GFP expression in the P cell and hyp7 decreased during mid-L1, but frequently increased late L1. At hatching no expression in observed in the V cells. In mid L1 the V cells divided and the anterior daughters begin to express GFP as they join the syncytium. Strong expression is also seen in the head and tail hypodermal nuclei of L1 larva. Expression in the hypodermal nuclei of older larva stages is similar to that in the L1. GFP expression decreases markedly in adults. GFP is also observed in other ectodermal cells. In L1, expression is observed in the G2(excretory pore) cell and in a cell tentatively identified as the W neuroblast. In older larva, expression continues in G2 but disappears in the W lineage once the cells divides to generate neurons. Beginning in L2, GFP is expressed in the region around the rectum. Expression is also occasionally observed in the anterior pharynx, in the nuclei with positions consistent with those of the pharyngeal epithelial cells. |
nuclei |
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Expr16
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Smudges of stain close to the surface of embryos - may be a non-specific (i.e. artefactual) pattern. Staining is diffuse, and seemingly not cell localised but present in the hypoderm. This type of pattern has been reported to be an artefact often seen when using the rol-6 gene as a cotransformation marker. This is the only instance of it in this screen, however, so it is included here as a 'possible' gene-specific expression pattern. |
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Other strain-- UL508 mid embryo + late embryo(author) = late cleavage stage embryo + elongating embryo + fully-elongated embryo(curator). |
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Expr125
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Expression is seen from embryos to adults in the hyp-7 nuclei. In embryos expression begins in the 200-300 cell stage and is usually comprised of two rows of 6 or 7 cells. In larvae, two rows of staining nuclei are seen down the length of the body. Staining is often restricted to the anterior half of the worm, with only a single row of staining nuclei in adults. |
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Expr1329
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UNC-42 expression was detected with a mouse antiserum in several cells as early as 260 minutes of embryogenesis and in neurons at high levels in the head by comma stage, the time when these neurons extend axons and establish connections. Expression in head neurons continues into adulthood. UNC-42 was strongly expressed in at least 20 pairs of neurons of the head, including the AVA, AVD and AVE interneurons, ASH sensory neurons, and RMD and SMB motor neurons. Other neurons that express high levels of UNC-42 include the AIN, AVH, AVJ, AVK, RIV, SAA and SIB interneurons. The same expression pattern in the head was detected in animals that carry a transgene that encodes an UNC-42 protein tagged at its C terminus with GFP(gmEx186). Antiserum to UNC-42 and the gmEx104 GFP reporter gene revealed low levels of unc-42 expression in hypodermis and additional neurons in the head. Transient expression of UNC-42 protein was also detected in the DD motor neurons at hatching and at low levels in postembryonic ventral cord motor neurons derived from P11. UNC-42 protein was not detected in descendants of P2-P10 or P12. In the tail, unc-42-gfp (gmEx104) is strongly expressed in PVT. |
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240 minutes of development (author) = late cleavage stage embryo (curator) Lineage expression: Rn descandents. |
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Expr1034
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Except for an earlier onset of GFP expression from both evpPRII.67 and evpPRII.75, all reporters displayed very similar expression patterns. Embryonic GFP expression reported by evpPRII.14 is first visible at approximately 240 minutes of development and is ubiquitous, a pattern that persists until hatching. At the beginning of each larval stage, dividing seam cells express mab-20::GFP, as do both daughter cells in males and hermaphrodites. After the anterior daughter fuses with surrounding hyp7, mab-20::GFP expression is downregulated or shut off in the both daughters. Other hypodermal expression is not evident in males or hermaphrodites. Other cells that express mab-20::GFP in hermaphrodites include the hermaphrodite-specific neurons at the L4 stage, vulva cells A to F throughout development, the migrating distal tip cells during the L4 stage, and several unidentified neurons within the nerve ring and ventral nerve cord. Expression in males include cells in the posterior ganglia, the migrating male linker cell beginning at the L3 to L4 transition, and the same nerve ring and ventral nerve cord expression observed in hermaphrodites. About 35 hours after hatching, mid-L3 males express mab-20::GFP in the 9 R(n) cells that give rise to the ray precursor clusters. Interior neuronal ganglia also express lower levels of mab-20::GFP at this stage. At 38-40 hours, when ray papillae are just visible, the 9 R(n)s and their descendents express mab-20::GFP. However, the underlying neural ganglia express the same level of GFP as the ray precursors at this stage. This pattern of expression continues until adulthood. |
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200- to 260-min-old embryos (Author) = late cleavage stage embryo (curator). |
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Expr952
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ldb-1b::gfp is expressed throughout the nervous system in larvae and in adults. Moreover it is expressed in developing and adult vulval cells and in adjacent developing and adult uterine cells. In adult male animals, all the neurons of the tail region also express ldb-1b::gfp. Expression analysis of the construct ldb-1a::gfp revealed a temporal and spatial overlapping expression pattern with ldb-1b::gfp. Additional expression is seen in all gonadal sheath cells and adult dorsal body muscle cells and vulval muscles. Embryonic expression of ldb-1 was detected first in 200- to 260-min-old embryos, in which all but the hyp-7 cells express the gfp construct. This ubiquitous expression becomes more restricted to the anterior, ventral, and posterior part in the comma stage embryo, in which all the cells but hyp-7 and the gut express the construct. |
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Among cells that express unc-129 promoter activity, only DA and DB motorneurons display morphological or axon guidance defects. |
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Expr1417
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Promoter activity was first detected at late gastrulation stage in cells that include descendants of the AB and E lineages. Expression continues through embryonic elongation in some of these cells. About 450 to 520 min after first cleavage, expression was observed in a subset of cells in the head, including one ventral muscle, and in all dorsal body wall muscles. Between 520 min and hatching, green fluorescent protein (GFP) expression was detected in the DA and DB classes of motorneurons, excluding DA8 and DA9. This pattern of expression persisted into the adult stage. In addition, expression was detected in a subset of cells in the head and in pharyngeal neurons and muscle. Of these, only interneuron I4 and muscle m8 were unambiguously identified. In late larval stages, expression was detected in the spermatheca, seam cells, CAN, PDE socket, and four cells that encircle the vulva. |
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Reporter gene fusion type not specified. |
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Expr1572
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Strong expression of let-502 was observed in lateral hypodermal seam cells at the onset of the elongation phase of morphogenesis.Other hypodermal cells in embryo showed weak expression. |
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Transgenic Marker: rol-6(su1006). |
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Expr517
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At approximately 260 min after the first cleavage, reporter gene expression is now also detected in nuclei on the dorsal surface of the embryo, corresponding to the position of the major hypodermal cells, and in six nuclei on each ventrolateral aspect of the embryo, corresponding to the position of the ventral hypodermal cells (P cells). Expression was first seen in granddaughters of C.paa and C.aaa about 240 min. after first cleavage. About 260 min. after first cleavage, expression was seen in dorsal and ventral hypodermal cells. Between comma-stage and 3-fold stage, expression was seen in dorsal and ventral hypodermal cells, and in tail and head hypodermis. After the 3-fold stage, expression becomes reduced, but is maintained a low levels in larvae and adults. After the 3-fold stage through adulthood, a digestive-tract component is expressed: probably vpi3D and vpi3V in the pharyngeal intestinal valve, virL and virR in intestinal rectal valve, and rect D, rect VL, rect VR. |
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Expr13488
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GFP::CED-11 was first detected in pre-bean-stage embryos, in which it was expressed broadly and had a diffuse cytoplasmic localization. By the twofold embryonic stage, GFP::CED-11 had a more restricted pattern inside the cell and also could be seen on the plasma membrane. GFP::CED- 11 was expressed in a few cells in the heads and tails of L1 larvae, where it was localized primarily to the plasma membrane. We detected GFP::CED-11 in puncta at the perimeter of apoptotic cells. |
