WormMine: ExpressionPattern Expr509

• Pattern: In the gut at the 8E cell stage; E descendants continue to show expression through late embryo. Pharyngeal cells derived from both AB and MS lineages show staining starting at comma stage. Intestinal and pharyngeal staining decline after hatching. The developing somatic gonad shows expression at L3 and L4. In comma to 1.5-fold stage embryos, hybridization was also seen in the tail region.
• Primary Identifier: Expr509
• Remark: For in situ hybridization, the signal from anti-sense probes was abolished by ribonuclease treatment of embryos. cDNA clone used as template for unidirectional PCR began at position 14 and ended at 2033 to remove the poly-A tract. For reporter gene, integrant gave same results, confirming that mosaicism. was not responsible for pattern. Reporter gene results gave resolution of cells. Transgenic Marker: rol-6(su1006). Reporter gene and in situ hybridization gave similar results, but lacZ expression was more intense in gut than pharynx. Hybridization results differed in being more intense in pharynx. early embryo(author) = late cleavage stage embryo(curator). late embryo(author) = comma + 1.5-fold + 2-fold + 3-fold + fully-elongated embryo(curator).

12 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
posterior region, from rectum to the end	tail		WBbt:0005741
organ producing either sperm or ova.	gonad		WBbt:0005175
embryonic cell	Eara		WBbt:0006161
embryonic cell	Eala		WBbt:0006104
embryonic cell	Eplp		WBbt:0006496
embryonic cell	Epra		WBbt:0006321
embryonic cell	Ealp		WBbt:0006546
embryonic cell	Eprp		WBbt:0006507
embryonic cell	Epla		WBbt:0006661
embryonic cell	Earp		WBbt:0006646

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004013	pha-4	F38A6.1	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes.		late cleavage stage embryo Ce	WBls:0000014