1 Children
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| nucleus of pedigree Cppppaa | Cppppaa nucleus | WBbt:0001868 |
4 Expression Patterns
| Remark | Reporter Gene | Primary Identifier | Pattern | Subcellular Localization |
|---|---|---|---|---|
| The expression of pha-1::lacZ with additional 2kb of 5'UTR showed identical expression pattern. | Expr1579 | The first expression of the pha-1:lacZ hybrid gene is detectable at the 100 cell stage of embryogenesis (130 minutes of embryonic development; all developmental times refer to time after the first cleavage at 20 degree) in cells that have positions in the embryo corresponding to the pharyngeal and body wall muscle precursors. Expression of the pha-1:lacZ hybrid gene at around 200 minutes of development is detected in about 25 cells that form a cylinder in the anterior part of the embryo. These pha-1:lacZ expressing cells are the pharyngeal precursors. The lineage of most of these cells (26 of 27) is restricted to pharyngeal fate and only the MSaaaaa cell produces pharyngeal cells and non-pharyngeal cells (two neurons of the ring ganglion). As soon as these 27 pharynx precursor cells divide (230-250 minutes), expression of the pha-1:lacZ gene in the pharyngeal precursors ceases and only a few cells in the presumptive pharynx region express the hybrid protein. At 420 minutes of development (2 fold stage) no pha-1:lacZ gene expression in the pharyngeal precursor cells is detectable. pha-1:lacZ expression was also observed in body wall muscle cell precursors. Expression of the pha-1 hybrid protein starts early in all body wall muscle cell precursors and is detectable throughout embryonic development in these cells. | ||
| 90 cell embryo(author) = 88-cell embryo(curator). CeMyoD :Basic Helix-loop-helix transcription factor. Related to the vertebrate MyoD family that is involved in the regulation of striated muscle cell fate.Vertebrate Homologs: Mammalian factors MyoD, MRF-4, Myf-5, myogenin.Invertebrate Homologs: Drosophila protein "Nautilus", sea urchin "SUM-1". Legacy Data: Author "Seydoux GC" "Krause MW". Date 1995-08. Function: Putative null mutation (cc450) of L. Chen and A.Fire suggests important role for CeMyoD in body wall muscle cell function and morphogenesis. CeMyoD is not required for bwm cell fate determination but is for proper bwm cell differentiation | Expr56 | Antibody staining: Transient nuclear staining in early MS lineage identical to lacZ pattern. Stable nuclear expression begins in the 2 daughters of D at the ~90 cell stage, then C and MS lineages that give rise to body wall muscle cells (bwm). The lone AB bwm appears positive after born. At pretzel stage, nuclear staining in the 6 GLR cells. Staining persists throughout postembryonic development in bwm cells, including post-embryonically born bwm. lacZ reporter gene expression: Multiple constructs with multiple lines. Transient expression in 2 MS daughters and the 4 MS granddaughters. Stable expression begins at ~90 cell stage in two daughters of D. Then on in C, MS lineages that give rise to body wall muscle precursors. At pretzel stage, hlh-1 is also expressed in the 6 GLR cells. positive hybridisation to RNA (in _situ) same as lacZ pattern except RNA appears to go away at bean stage and reappear later in embryogenesis | ||
| Other strain-- UL123 | Expr103 | This strain exhibits strong expression in the embryo. Expression is first seen in the 50-80 cell embryo and extends through to adulthood. It appears that most of the AB cells in the embryo stain, and what appears to be the cells of the C lineage. Some embryos exhibit staining in the two rows of nuclei that are the E lineage. All embryonic staining is very intense, and it spreads to the cytoplasm giving blue embryos, therefore obscuring the DAPI staining, making it difficult to count the number of cells in the embryos as each component begins expressing. This intense staining fades as the embryo ages, sometimes leaving blue comma stage embryos with no distinct nuclei staining. Hypodermal expression is seen in the 3 fold stage of embryogenesis and in young larvae which most probably are C-derived hyp-7 nuclei. Expression weakens as the worm gets older and is much less frequently expressed in adults. Some adults do show staining in the anterior hypodermal nuclei (hyp-3, hyp-4) and in the anterior hypodermal seam cells, also some nuclei stain in the tail. | ||
| 50-70 cell embryo(author) = 51-cell embryo(curator). No maternal RNA. | Expr569 | Staining in body muscle precursors at 60-cell through bean embryo. |
11 Life Stages
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. | embryo Ce | WBls:0000003 | ||
| The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. | fully-elongated embryo Ce | WBls:0000021 | ||
| The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over. | elongating embryo Ce | WBls:0000015 | ||
| The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing. | enclosing embryo Ce | WBls:0000013 | ||
| The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over. | proliferating embryo Ce | WBls:0000004 | ||
| The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo. | comma embryo Ce | WBls:0000017 | ||
| The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo. | 2-fold embryo Ce | WBls:0000019 | ||
| The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage. | 3-fold embryo Ce | WBls:0000020 | ||
| The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo. | 1.5-fold embryo Ce | WBls:0000018 | ||
| The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage. | bean embryo Ce | WBls:0000016 | ||
| The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes. | late cleavage stage embryo Ce | WBls:0000014 |
4 Parents
| Definition | Name | Synonym | Primary Identifier |
|---|---|---|---|
| cell that exists in and is part of an embryo. | embryonic cell | WBbt:0007028 | |
| embryonic cell | Cppppa | WBbt:0006263 | |
| embryonic body wall muscle cell derived from C lineage. | body wall muscle cell from C lineage | WBbt:0006806 | |
| Body wall muscle of dorsal right quadrant medial row. | DMR muscle | WBbt:0008264 |
