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Fusion junction is at position 21392 in F18G5 ...GTATTCTCTGAGAGAAGGAATGATC/lacZ Young and Hope (1993). Dev. Dynam. 196:124-132 = [cgc1752]. Legacy Data: Author "Arnold JM" "Guo J" "Hope IA"Date 1992-01. life_stage summary : postembryonic |
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Expr51
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b-galactosidase expression in the spermathecae and the three rectal epithelial cells. Staining in the rectal area was first observed in L1 larvae whilst expression in the spermathecae appeared as the structure formed in L4 larvae. Variable staining was also seen throughout the uterus. In the mature gonad staining appeared to be in the two sets of two large toroidal epithelial cell, Ut-1 and Ut-2. Staining was also observed in the large, multinucleate H-shaped Use cell which attaches the uterus to the seam cells and to the four epithelial cells Ut-1 and Ut-2. The Uv-1 cells did not appear to stain. Individual worms often showed only one component of this expression pattern. In the male, expression was observed to be dispersed in what appeared to be all or part of the proctodeum. |
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Expr1164
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Transgenic animals that carry this construct show GFP expression in a variety of tissue types. GFP expression is observed in the intestine, and the posterior cells express GFP more intensely than the remaining intestinal cells. Other cells include the rectal epithelial cells, the pharynx, the somatic gonad, and vulval hypodermal cells. In addition, the IP3 receptor is expressed in hypodermal cells of the tail, rectum, and head. Pharyngeal expression is restricted to the muscles of the metacorpus, isthmus, and the anterior portion of the terminal bulb (m4, m5, and m6). This construct was only expressed in neurons LUA and PDA. GFP is expressed in the gonad sheath cells, spermatheca, spermathecal valve, and uterine sheath cells. GFP is expressed in the vulval hypodermal cells. |
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Expr3206
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In addition to expression in a number of neurons (RMEL/R, RID, BDUL/R, AIYL/R, AVHL/R, AIZL/R, ALNL/R, RICL/R, RIAL/R and PDA) as has been reported previously, intense fluorescence was also observed in uterine toroid cells (ut1 and ut2). |
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Expr1816
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All three reporter constructs were expressed in all major contractile tissues, starting during embryogenesis and continuing until adulthood. Fluorescence was particularly pronounced in striated muscle (body wall muscles used for locomotion), non-striated muscle (pharyngeal muscles used for pharyngeal pumping, vulval and uterine muscles used for egg laying, the sphincter muscle and anal depressor used for defecation), and in myoepithelial cells (gonadal sheath cells used for ovulation). Furthermore, all three constructs showed expression in the intestine. Some cells expressed CeSERCAb::GFP but not CeSERCAa::GFP. These include the somatic cells of the spermatheca and the excretory canal and the uterine sheath cells. No cells were found that expressed CeSERCAa::GFP but not CeSERCAb::GFP. |
Fluorescence from Psca-1::GFP was evenly distributed over the cytosol. However, both CeSERCAa::GFP and CeSERCAb::GFP showed specific subcellular localization. Both fusion proteins were found in a tubular meshwork that was most distinct in the body wall muscle cells. This staining was continuous with staining at or near the dense bodies, structures functionally analogous to vertebrate Z-lines. In body wall muscle cells and other cells, staining was also often localized in internal vesicles and membrane-like structures, including the nuclear envelope. |
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In general, the expression patterns found for the translational fusion constructs were similar to those reported above for the transcriptional fusions. |
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Expr2245
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NHX-3 appeared to be associated with intracellular membranes. NHX-3::GFP expression was identical in both translational and transcriptional fusions and occurred primarily in the hypodermal cells of the main body syncytium; in addition, in adult animals the uterine cells in the region closest to the vulva were intensely labeled, as were the spermathecal junction cells. The uterine cells are likely ut1, a toroidal cell proximal to the vulva. In addition, labeling was detected in socket cells and the excretory pore cell. The fluorescent expression pattern resembled dots distributed randomly throughout the cells. Under maximum resolution, these structures were seen to be elliptical, with circumferential labeling. In contrast to the usual suspects (Golgi, endoplasmic reticulum, endosomes, or mitochondria) these dots may represent storage granules, because hypodermal cells function as a major storage depot for granules and lipid droplets. Alternatively, the structures could be one of two other organelles that are unique to hypodermal cells, multivesicular bodies, or Ward bodies, which presumably play roles in the hypodermis-specific functions of secretion of cuticle components or the phagocytosis of apoptotic cells. |
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To cite this work: Ghosh, Srimoyee; Inoue, Takao; Sternberg, Paul (2015): ina-1::gfp expression. WormBase. Dataset. DOI: http://dx.doi.org/10.17912/W2159J . Reviewed by Bhagwati Gupta. Funding: National Institutes of Health USPHS training grant GM07616. |
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Expr12292
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ina-1 is expressed throughout uterine toroids. At mid L4, expression is seen in uterine toroid 1 and 4 (ut1, ut4) and in the anterior uterine toroids 2 and 3 (ut2, ut3). In addition, ina-1::GFP is detected in the spermathecal-uterine junction and spermatheca. At the L4 lethargus stage, bright expression is observed in uterine toroids 1 and 4, the spermathecal-uterine junction, and spermatheca. Dimmer expression is present in uterine toroid 2 and posterior toroid 3. Fluorescence is also detected in vulE. |
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