WormMine: ExpressionPattern Expr1317

• Pattern: The expression of lim-6 in all neurons continues throughout adulthood. The expression of lim-6 in the uterus is dynamic. lim-6 reporter gene constructs (lim-6prom::GFP, lim-6up::GFP, lim-6r::GFP) start to be expressed in two uterine cells during the late L3/early L4 stage and the expression widens during the L4 stage to include the uv2 and uv3 cells, several uterine toroid (ut) cells, which form the lumen of the uterus and at least one cell type (sujn) of the spermatheca-uterine junction. Occasionally, weaker and less consistent expression can be observed in some cells of the distal side of the spermatheca, which connect the spermatheca to the rest of the somatic gonad. The lim-6r::GFP fusion gene reveals expression in restricted set of neurons, epithelial cells of the uterus and the excretory system. Reporter gene expression in the nervous system begins late in embryogenesis at about 300 minutes of development, which is after these neurons have been generated and while they initiate neurite outgrowth. After hatching, lim-6r::GFP is expressed in one chemosensory neuron, ASEL, and in eight inter- and motorneurons. Most of these neurons are GABAergic, namely RMEL/R, AVL, RIS and DVB. RMEL/R, AVL and DVB are motorneurons, whereas RIS is an interneuron. The other three neurons, PVT and RIGL/R, express the neuropeptide FMRFamide.
• Primary Identifier: Expr1317
• Remark: Reporter gene fusion type not specified.

15 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A valve tissue that connects the spermatheca and the uterus of a hermaphrodite gonad.	spermathecal-uterine junction	sp-ut valve	WBbt:0006756
Neuron class of one interneuron, projects along ventral cord to ring.	PVT	lineage name: ABplpappppa	WBbt:0004070
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASEL	lineage name: ABalppppppaa	WBbt:0003904
	excretory system		WBbt:0005736
Neuron class of one ring interneuron, cell body in dorsorectal ganglion, inervates rectal muscles.	DVB	lineage name: K.p	WBbt:0004822
Neuron class of one ring and ventral cord interneuron, few synapses.	AVL	lineage name: ABprpappaap	WBbt:0003843
Ring motor neuron	RMEL	lineage name: ABalaaaarlp	WBbt:0005025
Ring motor neuron	RMER	lineage name: ABalaaaarrp	WBbt:0005023
Neuron class of one ring interneuron.	RIS	lineage name: ABprpappapa	WBbt:0005045
uterine-vulva cell, distal (of three ut cells) to the vulva.	uv3		WBbt:0006793
uterine-vulval cell, medial position of three ut cells.	uv2		WBbt:0006792
toroidal epithelial cell that make up the bulk of the uterus, four cells anterior and four cells posterior to the vulva.	uterine toroidal epithelial cell	ut cell	WBbt:0006784
Ring interneuron	RIGR	lineage name: ABprppappaa	WBbt:0003930
Ring interneuron	RIGL	lineage name: ABplppappaa	WBbt:0003932

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002988	lim-6	K03E6.1	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	A developemental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until death.		postembryonic Ce	WBls:0000022