WormMine: ExpressionPattern Expr14584

• Pattern: In hermaphrodites, swm-1 was expressed in posterior body wall muscle consistent with the non-sex-specific nature of this tissue. SWM-1::mCherry protein was detectable in muscle and secreted into the body cavity, as evidenced by its presence in coelomocytes. Interestingly, swm-1 also was expressed in the spermathecae, structures that are unique to the hermaphrodite gonad, and SWM-1::mCherry surrounded sperm that were stored in the spermathecal lumen. Indeed, SWM-1::mCherry protein was present throughout the outer hermaphrodite reproductive tract and it surrounded fertilized eggs. As it can be difficult to distinguish between the body cavity and the uterus, we used GFP-labeled seminal fluid to mark the lumen of the uterus. jn62[swm-1::mCherry] hermaphrodites mated to males that expressed TRY-5::GFP showed clear colocalization of SWM-1::mCherry and TRY-5::GFP in the uterus. In similar experiments, we found that muscle-derived SWM- 1::mCherry also colocalized with GFP transferred in seminal fluid. Thus, as in males, SWM-1 is secreted into the hermaphrodite body cavity and then taken up into the gonad, in which it is present throughout the sperm migratory path.
• Primary Identifier: Expr14584

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
	reproductive tract		WBbt:0005744

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00023417	swm-1	C25E10.9	Caenorhabditis elegans

0 Life Stages