WormMine: ExpressionPattern Expr8160

• Pattern: The reporter was widely expressed and the expression level was dynamic, varying among tissues and developmental stages. The expression of fli-1::gfp was first detected during embryogenesis before the apparent morphogenesis occurred. In the comma-stage embryo, expression of fli-1::gfp was detected in muscle cells. The expression of fli-1::gfp was not observed before the 32-cell-stage embryos (n > 40 embryos examined). At post-embryonic stages, expression of fli-1::gfp was detected in many tissues, including the pharyngeal muscle, rectum muscle, vulva muscle, proctodeum muscle in males, and somatic gonad tissues (including spermatheca and distal tip cell).
• Primary Identifier: Expr8160
• Remark: Picture: Figure 9.
• Subcellular Localization: The GFP was localized mainly in the cytoplasm. The fli-1 expression in the spermatheca was unevenly distributed with strong expression in the distal and proximal part of spermatheca. fli-1 was also expressed within neuronal migratory structures such as axons. In the body-wall muscle, fli-1::gfp was localized in a distinct striated pattern, with accumulation of FLI-1::GFP at dense bodies, which is similar to the Z-disc in the muscle cells in other organisms. The expression pattern of fli-1 in body-wall muscle cells is reminiscent of the distribution of the actin filaments. Authors stained F-actin in body-wall muscle cells with rhodamine-labeled phalloidin and found that it colocalized with FLI-1::GFP.

11 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
Anterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	anterior distal tip cell	gon herm dtc A	WBbt:0004520
Posterior distal tip cell, inhibit meiosis in neighbouring germ cells, lead gonad during morphogenesis, herm gonad	posterior distal tip cell	gon herm dtc P	WBbt:0004506
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292
a saddle-shaped muscle cell that encircles the intestinal-rectal valve	anal sphincter muscle	lineage name: ABprpppppap	WBbt:0005798
	spicule retractor muscle		WBbt:0005827
	spicule protractor muscle		WBbt:0005826
	gubernacular retractor muscle		WBbt:0005825
	gubernacular erector muscle		WBbt:0005824

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001443	fli-1	B0523.5	Caenorhabditis elegans

10 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004