WormMine: ExpressionPattern Expr10610

• Pattern: Transgenic animals expressing green fluorescent protein (GFP) under the control of a fully rescuing sphk-1 promoter fragment displayed fluorescence in multiple tissues, including the hypodermis (skin), muscles, the intestine, excretory canal cells, and some neurons in the head and tail.In DA/DB class cholinergic motor neurons, functional GFP-tagged SPHK-1 fusion proteins (SPHK-1-GFP) were detected in extranuclear regions in cell bodies, likely corresponding to Golgi (Sumakovic et al., 2009), occasionally in puncta in dendrites (data not shown), and in puncta at en passant presynaptic terminals (Chan et al., 2012). Colocalization studies revealed that SPHK-1-GFP was closely apposed to but not colocalized with the synaptic vesicle (SV) and actin-associated protein SNN-1/synapsin. In body wall muscles, SPHK-1-GFP was primarily observed on the outer surface of mitochondria as demonstrated by its colocalization with an RFP-tagged outer membrane mitochondrial marker. Thus, SPHK-1 appears to localize to different cellular membranes depending on cell type.SPHK-1 associates with presynaptic membranes at release sites, but not synaptic vesicles.
• Primary Identifier: Expr10610

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
Neuron class of seven motoneurons that innervate dorsal muscles and have cell bodies in the ventral cord.	DB neuron		WBbt:0005274
Neuron class of nine ventral cord motor neuron, innervates dorsal muscle.	DA neuron		WBbt:0005278
neuron with its cell body situated in the tail, posterior to rectum.	tail neuron		WBbt:0006759
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007918	sphk-1	C34C6.5	Caenorhabditis elegans

0 Life Stages