WormMine: ExpressionPattern Expr3187

• Pattern: Transgenic worms containing the pmr-1::gfp construct showed GFP expression beginning as early as 3-fold stage and continued to be expressed throughout larval and adult stages. Expression was spatially confined to hypodermal seam cells, spermatheca, and intestine. When pPMR pro & cDNA::gfp was injected, transgenic worm showed essentially the same expression pattern, but different subcellular localization. Taken together, CePMR-1 is expressed in hypodermal seam cells, intestinal cells and spermatheca and male gonad; subcellularly it is localized at Golgi complex.
• Primary Identifier: Expr3187
• Subcellular Localization: The GFP was found to be highly diffused in the worms harboring pPMR pro::gfp, but in worms expressing pPMR pro & cDNA::gfp, GFP appeared to be punctated and was confined to cytosolic region. This difference is likely to reflect the differences in the sub-cellular localization. pPMR pro::manII DsRed was injected into the transgenic worm already expressing CePMR-1::GFP fusion protein. The pPMR pro::manII DsRed, which encodes a part of alpha mannosidase II (a Golgi resident protein), was expressed under the control of pmr-1 promoter. The localization of CePMR-1 is completely superimposable with that of mannosidase, confirming the localization of CePMR-1 at Golgi apparatus. Similar pattern was observed in intestine and spermatheca.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
gonad of a male animal, produces sperm.	male gonad		WBbt:0006794

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004063	pmr-1	ZK256.1	Caenorhabditis elegans

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020