WormMine: ExpressionPattern Expr14940

• Pattern: All five tagged alleles showed expression in two areas: the distal germline and spermatheca. Signal in the spermatheca was strongest at the membrane but also sometimes visible at a lower level in spermathecal nuclei. Expression in the distal germline was present in both sexes and strongest in the distal-most ~20 rows of germ cells, becoming weaker more proximally. Germ cells in the distal-most gonad showed membrane staining similar to that seen with antibodies to the extracellular domain of GLP-1 (e.g. Crittenden et al., 1994); in addition, staining in the nucleus was detected, but at a low level compared to membrane staining. Expression in L4 and adult animals was not observed outside the gonad or in the male somatic gonad (although we cannot exclude the possibility of expression below our threshold of detection). We also examined pre-gastrulation embryos and observed both membrane and nuclear expression of tagged GLP-1, in a pattern consistent with GLP-1 expression in the AB cell lineage, as reported previously (Evans et al., 1994; Priess, 2005).
• Primary Identifier: Expr14940

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850
Multi-cellular organism that can produce both male and female gametes.	hermaphrodite	hermaphroditic organism	WBbt:0007849
A cell that is the AB blast cell or is a lineal descendant of AB.	AB lineage cell		WBbt:0008611

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001609	glp-1	F02A9.6	Caenorhabditis elegans

0 Life Stages