WormMine: ExpressionPattern Expr1325

• Pattern: The GFP::TLN-1 reporter showed prominent expression in all differentiating vulval and uterine cells. In addition to the basal and lateral plasma membrane- associated signal, GFP::TLN-1 expression was also observed in the cytoplasm of the vulval cells of wild-type L3 larvae. lin-26 transcripts were first weakly detected by in situ hybridization in the germline precursors (P3, P4 and Z2/Z3) until the 50-cell stage and occasionally in their sisters. After the 100-cell stage, lin-26 transcripts and the beta-galactosidase encoded by the lin-26::lacZ construct were detected in hypodermal precursors, then in differentiating hypodermal and support cells. The only difference between in situ hybridization and antibody staining is that lin-26 transcripts could not be detected in body hypodermal cells after the 350-cell stage nor in any cell after the comma stage, whereas LIN-26 protein can be detected until the end of embryogenesis in all hypodermal cells and in all support cells.
• Primary Identifier: Expr1325
• Remark: Reporter gene fusion type not specified.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
embryonic founder cell	Psub3	lineage name: P0.ppp	WBbt:0006875
Embryonic founder cell of germ line	Psub4	lineage name: P0.pppp	WBbt:0004421
Germ line precursor cell	Z2	lineage name: P4.p	WBbt:0004576
Germ line precursor cell	Z3	lineage name: P4.a	WBbt:0004575
Constituent cell of a hermaphrodite vulva.	vulval cell	vulval epithelial cell	WBbt:0007831
cell of the epithelia of uterus.	uterine epithelial cell		WBbt:0008435

2 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003012	lin-26	F18A1.2	Caenorhabditis elegans
WBGene00006771	tln-1	Y71G12B.11	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003