WormMine: ExpressionPattern Expr9877

• Pattern: CEH-63 expression was observed in DVC and the uterus. The GFP signal was localized to the cell nuclei, presumably due to the DNA binding activity of the transcription factor taking the fusion protein to the nucleus. The nuclear localization allowed identification of the uterine cells expressing the CEH-63 C-terminal GFP fusion as ut2 and ut3. This identification was made in young adults after uterine development was complete. Although no extra expression was observed for the N-terminal fusion, for the C-terminal fusion there was also reliable expression in 6-10 nuclei in the head region and at a similar level to that in DVC. There was also weak but frequent expression of the C-terminal fusion in two other unidentified nuclei, posterior to DVC, in the hermaphrodite tail. CEH-63 expression was observed in DVC and the uterus. The GFP signal was localized to the cell nuclei, presumably due to the DNA binding activity of the transcription factor taking the fusion protein to the nucleus. The nuclear localization allowed identification of the uterine cells expressing the CEH-63 C-terminal GFP fusion as ut2 and ut3. This identification was made in young adults after uterine development was complete. Although no extra expression was observed for the N-terminal fusion, for the C-terminal fusion there was also reliable expression in 6-10 nuclei in the head region and at a similar level to that in DVC. There was also weak but frequent expression of the C-terminal fusion in two other unidentified nuclei, posterior to DVC, in the hermaphrodite tail.
• Primary Identifier: Expr9877
• Reporter Gene: [CEH-63::gfp] translational fusion.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
posterior region, from rectum to the end	tail		WBbt:0005741
anterior-most body region containing the pharynx.	head		WBbt:0005739
uterine toroidal epithelial cell.	ut2		WBbt:0006786
Neuron class of one ring interneuron, cell body in dorsorectal ganglion.	DVC	lineage name: Caapaa	WBbt:0004821
uterine toroidal epithelial cell.	ut3		WBbt:0006787

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00045215	ceh-63	C02F12.10	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023