WormMine: ExpressionPattern Expr2992

• Primary Identifier: Expr2992
• Remark: Transgenic lines (n = 4) were made in the wild-type N2 background, harboring a shorter GFP fusion protein including only the first 144 amino acids of UNC-95, which corresponds to the predicted su33 allele, Ex[unc-95(su33)::GFP; rol-6]. Low GFP expression was detected in the dense bodies and M-lines of body wall muscles; however, there was no expression in the muscle-muscle cell boundaries. Weak expression was also detected in muscle nuclei. In addition, the expression was not equal along the body wall muscle cells and appeared in a mosaic pattern. In several cells (observed in all lines), the protein accumulated to very high levels that were not observed in the wild-type UNC-95 construct. In those cells, the organization of the M-lines and dense bodies was disrupted, and these structures appeared as intense stripes or aggregates. On the other hand, additional surrounding cells had no GFP expression at all.
• Subcellular Localization: Strong GFP expression was observed at the embryonic stage 350 min after the first cell division. The staining is dispersed in the muscle cell cytoplasm as was also shown for vinculin and integrin at this stage of muscle development, when muscle cell precursors are positioned in lateral rows adjacent to the seam cells. The staining gradually becomes more localized at the membrane until the muscles are fully positioned into quadrants and the expression is shown in two separate parallel stripes. Later on the expression becomes localized to the discrete spots of the newly formed muscle dense bodies, but through all stages GFP is also present in the cytoplasm. Nuclear localization of UNC-95 could be clearly observed from the threefold stage. In adult animals, UNC-95 is expressed in body wall muscles in the dense bodies, M-lines, and muscle-muscle cell boundaries. Strong expression is also detectable in muscle cell cytoplasm and nucleus. In the vulval muscles the protein is strongly localized at sites of muscle attachment to the hypodermis. Expression was also detected in the anal depressor muscle. A bright ring at the tip of the buccal cavity seems to be the attachment between the arcade cells to the hypodermal anterior hyp1 cell, the pharyngeal epithelium, and the associated cuticles. Thus, in different expressing cells UNC-95 is mainly localized to the cellular attachment sites between the cell membrane and the ECM.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006824	unc-95	Y105E8A.6	Caenorhabditis elegans

0 Life Stages