WormMine: ExpressionPattern Expr11648

• Pattern: Similar to transgenic animals expressing a Psup-10::gfp translational fusion transgene, Psup-18::gfp transgenic animals displayed GFP fluorescence in body-wall, defecation and vulval muscles. In body-wall muscle cells, the SUP-10::GFP and SUP-18::GFP fusion proteins both localized to cell-surface regions aligned with dense bodies, the functional analogs to vertebrate Z-lines that connect the myofibril lattice to the cell membrane. In addition to muscles, three neurons in the head of Psup-18::gfp transgenic animals also displayed GFP staining. The authors previously reported expression of a Psup-9::gfp reporter in the four SIA interneurons. They stained the Psup-18::gfp transgenic animals with an anti-CEH-17 antibody, which labels the four SIA neurons and the ALA neuron, and found that the neurons expressing the SUP-18::GFP fusion protein were not the SIAs. SUP- 10 and SUP-18 colocalize in subcellular structures, including the dense bodies in the body-wall muscles. Since GFP fusions to SUP-9 and UNC-93 localize similarly, this result suggests that SUP-18 colocalizes with a SUP-9/UNC-93/SUP-10 complex.
• Primary Identifier: Expr11648

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292

2 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006319	sup-10	R09G11.1	Caenorhabditis elegans
WBGene00015334	sup-18	C02C2.5	Caenorhabditis elegans

0 Life Stages