WormMine: ExpressionPattern Expr16536

• Pattern: Short isoform. We generated strains that express either long(ex7+) or short(ex7-) MBL-1 protein isoforms, tagged with mCherry, under both endogenous promoters together, and investigated their subcellular localization at the L4 larval stage (when most tissue development is complete). Expression of MBL-1 was detected in various tissues in a pattern consistent with previous studies [38, 39], specifically in the head and tail ganglia, the ventral nerve cord (VNC), the spermatheca, and the posterior gut. Consistent with the human longer MBNL isoform, mCherry signals showed a distinct nuclear localization when linked with long(ex7+) isoforms, most noticeably in, but not limited to, cells of the gut and the head region. In contrast, short(ex7-) isoforms showed a more diffuse pattern, consistent with localization both in the nucleus and cytoplasm. We note that the expression in the gut might be ectopic and a possible consequence of the presence of the unc-54 30-UTR, as reported before [43].
• Primary Identifier: Expr16536

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
ganglion in the head.	head ganglion		WBbt:0005135
ganglion in the tail region (posterior to rectum).	tail ganglion		WBbt:0006977

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00019347	mbl-1	K02H8.1	Caenorhabditis elegans

0 Life Stages