WormMine: ExpressionPattern Expr11536

• Pattern: The integrated pRIC-19::GFP reporter is expressed at high levels in the M2 neurons. Neurons of the head ganglion individually express comparatively low levels of pRIC-19::GFP in their cell bodies but, collectively, create a bright halo surrounding the pharynx. The earliest signs of expression are at approximately 450 min of development, when the pharynx primordium has already elongated and begun to form the two muscular bulbs. At this stage and until late in embryogenesis, expression of the pRIC-19::GFP reporter is present in one unidentified unbranched cell within the metacorpus. This cell may be MS.paapaap, the sister cell of M1 (M1 is the only other pharyngeal cell besides the M2's that expresses the pRIC-19::GFP reporter in the adult). Consistent with the disappearance of the GFP-positive cell in the metacorpus in late L1, MS.paapaap is a cell programmed to undergo apoptosis during development (Sulston et al., 1983). Other unique cells located in the metacorpus are the motor neuron M4, the interneuron I3, and the motor-interneuron MI. The three-fold stage is the earliest stage where the M2 axons can be unambiguously visualized using the pRIC-19::GFP reporter. At the three-fold stage, the motorneurons M1 also express considerable levels of the pRIC- 19::GFP reporter but during late embryogenesis and beyond the L1 larval stage, expression level remains high only in the M2 cell, fades away completely in the unidentified pharyngeal cell located in the metacorpus, and retains only weak expression in the cell M1.
• Primary Identifier: Expr11536
• Remark: Temporal description.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
ganglion in the head.	head ganglion		WBbt:0005135
Pharyngeal motorneurons	m2L	lineage name: ABaraapappa	WBbt:0004487
Pharyngeal motorneurons	m2R	lineage name: ABaraappppa	WBbt:0004473
Neuron class of one pharyngeal motorneuron.	M1 neuron	lineage name: MSpaapaaa	WBbt:0004488

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004368	ric-19	C32E8.7	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018