WormMine: ExpressionPattern Expr12001

• Primary Identifier: Expr12001
• Subcellular Localization: LIN-41 is strongly expressed in adult hermaphrodite germ cells beginning in mid-pachytene. LIN-41 protein levels rise dramatically at this time, increasing several-fold over a distance corresponding to just a few cell diameters. The timing of this rise consistently occurs just before the peak of MPK-1 mitogen-activated protein kinase (MAPK) activation in mid-pachytene, which may promote the progression of oogenic germ cells from early to late pachytene (Lee et al. 2007). LIN-41 is also abundantly expressed in diplotene and diakinesis-stage oocytes, but is generally reduced in abundance in the -1 oocyte as it undergoes meiotic maturation (14/17 oocytes identified), suggesting that LIN-41 may be eliminated at this time. Consistent with this interpretation, an N-terminal GFP::LIN-41 fusion protein, generated by CRISPR-Cas9 genome editing of the endogenous locus, is expressed in oocytes, disappears during meiotic maturation, and is absent from early embryos. Most LIN-41 is diffusely cytoplasmic, with a small amount of punctate LIN-41 that is most evident during pachytene. LIN-41 was also not detected in the germ lines of males or mid-L4 stage larvae, both of which have late pachytene-stage germ cells undergoing spermatogenesis, suggesting that pachytene-stage LIN-41 expression is restricted to germ cells committed to oogenesis. The primary, and possibly exclusive, site of LIN-41 expression in adults appears to be the oogenic germ line. LIN-41 expression in the C. elegans germ line is strongly associated, both developmentally and spatially, with the oogenic fate.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
a cell, of either sex, directly concerned in the production of a new organism.	germ cell		WBbt:0006796
Multi-cellular organism that can produce both male and female gametes.	hermaphrodite	hermaphroditic organism	WBbt:0007849

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003026	lin-41	C12C8.3	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A developmental life stage of the nematode Caenorhabditis elegans that occurs in an egg (oocyte), before fertilization by sperm.		unfertilized egg Ce	WBls:0000669