WormMine: AnatomyTerm WBbt:0004757

• Definition: Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic
• Name: HSNR
• Primary Identifier: WBbt:0004757
• Synonym: lineage name: ABprapppappa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABprapppappa	ABprapppappa nucleus		WBbt:0002089

0 Expression Clusters

206 Expression Patterns

• Primary Identifier: Expr4858
• Pattern: A-class motor neuron: enriched in embryo (2.8) and larva (3.1). Neuronal expression include: DA, VB, AS, DB, DD, HSN, VC4&5, AIY, head neurons. Also expressed in other cells: Muscle, intestine. Pan-neuronal: expressed in embryo; enriched in larva (1.5).

• Primary Identifier: Expr4826
• Remark: Picture: fig. S3 A.
• Pattern: A transgene containing the sel-10 promoter driving green fluorescent protein (GFP) labeled a subset of neurons including the HSNL, which suggests that sel-10 is expressed in the HSNL. sel-10 promoter driven GFP is widely expressed in body wall muscles (not consistent though), intestine(not consistent), pharynx, distal tip cell, spermatheca, and within nervous system, it is expressed in HSNs, some ventral cord neurons, a bunch of unidentified head neurons and a few tail neurons. --Pers. Comm. from Mei Ding 11-20-07.

• Primary Identifier: Expr4900
• Remark: Picture: Figure 4.
• Pattern: UNC-69::GFP expression was first detectable in embryos. In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. In older larvae and adults, UNC-69::GFP was expressed in neurons of the anterior, lateral, ventral and retro-vesicular ganglia in the head, and in neurons of the preanal, dorso-rectal and lumbar ganglia in the tail. The fusion protein was also present in the ventral nerve cord (VNC), in the dorsal nerve cord (DNC), in the dorsal and ventral sublateral nerve cords, and in commissural axons. The reporter was expressed in the neurons named CAN, HSN, ALM, PLM, AVM, PVM, BDU, and SDQR, as evidenced by its localization to the cell bodies of these neurons. Expression of unc-69 in these latter cells was confirmed using an unc-69::LacZ::NLS fusion.
• Subcellular Localization: In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. In older larvae and adults, UNC-69::GFP was expressed in the cell bodies of neurons.

• Primary Identifier: Expr4590
• Pattern: Promoter activity with distinct GFP expression in lateral hypodermal seam cells and in the non-seam cell hypodermis was observed throughout development. Additionally, expression was noted in many neurons, including the ventral nerve cord (VNC) and the hermaphrodite specific neuron (HSN). GFP was also observed in various somatic gonad tissues including the anchor cell in larval stages and adult vulval muscle cells, the distal tip cells, a subset of the vulval precursor cells, uterine cells, and spermatheca.

• Primary Identifier: Expr8656
• Remark: Picture: Fig 3.
• Pattern: The fusion protein encoded by the transgene was preferentially detected in several tissues including the nervous system. However, its presence was not uniform. Within the nervous system, ELPC-1::GFP was seen predominantly in a pair of neurons that control egg-laying, the HSNs, and in chemosensory neurons in the head. Within the latter class of neurons, the ELPC-1::GFP level was particularly high in the ASE, ADF and ASK pairs of neurons. Outside of the nervous system, a strong ELPC-1::GFP signal was seen in the pharynx and the vulva. In all animals examined, ELPC-1::GFP expression was also seen in the two CAN cells.
• Subcellular Localization: In the nervous system, expression was seen both within the cell bodies and along the entire lengths of the neuronal processes. Outside of the nervous system, in all cells in which ELPC-1::GFP was seen, fluorescence was restricted to the cytoplasm.

• Primary Identifier: Expr9674
• Pattern: Pprkl-1::GFP was only consistently detected in VC1-6 and the HSN neurons and a small subset of neurons in the head and ventral nerve cord. In non-neuronal tissue, prkl-1 promoter activity was detected in somatic gonad cells, uterine cells, distal tip cells, and vulval cells.

• Primary Identifier: Expr13357
• Pattern: Peel-1::GFP was observed in non-neuronal tissue (pharynx, intestine, and vulva) and was strongly expressed in neurons, including numerous neurons in the head, motor neurons, mechanosensory neurons, HSN neurons, and tail neurons. GFP was detected in cholinergic and GABAergic motor neurons. No expression was observed in muscle.

• Primary Identifier: Expr8970
• Remark: Picture: Fig 7.
• Pattern: GFP expression was observed in several neurons including head neurons, motor neurons located in the ventral nerve cord, HSN and CAN neurons, and tail neurons. However, rep-1 does not seem to be expressed in all the neurons. Not only was GFP involved in the neuronal expression, it was also expressed in various muscles such as body-wall, pharyngeal, intestinal and anal sphincter, in addition to the seam cells, hypodermis and the intestine.

• Primary Identifier: Marker49
• Remark: Picture: N.A. Reporter gene fusion type not specified.
• Pattern: Expressed in anterior neurons, including AIY, AIZ, RID, M5, ASI, and labial sensory neurons, VNC motorneurons, midbody neurons HSN, CAN, and PVM, tail neurons DVB, DVC, and PDB, and the nonneuronal excretory cell, uterine muscles. -- according to pers. comm. from Oliver Hobert.

• Primary Identifier: Marker88
• Remark: Picture: N.A.
• Pattern: Marker for HSN neurons.

• Primary Identifier: Marker79
• Remark: Picture: Figure 2. Reporter gene fusion type not specified.
• Pattern: Expressed in HSN neurons.

• Primary Identifier: Expr15558

• Primary Identifier: Expr1688
• Remark: The cellular expression pattern for nlp-3 fragments was indistinguishable from the cellular expression pattern for nlp-3 subcloned constructs. Transgenic lines were generated by coinjection of the nlp::gfp construct (5070 ng/ul) and lin-15 rescue construct (pJM24,100 ng/ul) into lin-15(n765ts) animals. At least two independent transgenic lines were analyzed for each nlp gene; 5-10 animals were scored per line. 1,1'-Dioctadecyl-3,3,3',3 -tetramethylindodicarbocyanine perchlorate (Molecular Probes) was used to stain amphid and phasmid neurons to facilitate identification.
• Pattern: Expressed in ADF, ASE, ASH, AWB, ASJ, BAG, HSN, I1, I2, I3, I4, MI, M3, NSMR, 3 head neurons, VNC, oocytes, I6, M2, pm1VL, intestine.

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr1124
• Pattern: Excretory cell, vulva, hermaphrodite-specific neurons, enteric muscles, first four epithelial cells of intestine, and the uterus.

• Primary Identifier: Expr8203
• Pattern: Expressed in DA, VB, AS, DB, DD, HSN, VC4 & 5, AIY, head neurons, muscles, intestine. acr-14::GFP is expressed in RME in the head and in ventral nerve cord (VNC) motor neurons.

7 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019

2 Parents

Definition	Name	Synonym	Primary Identifier
embryonic cell	ABprapppapp		WBbt:0006743
Neuron class of two motoneurons that innervate vulval muscles in the hermaphrodite.	HSN	hermaphrodite specific neuron	WBbt:0006830