WormMine: ExpressionPattern Expr4493

• Pattern: The embryos near the vulva assumed stronger fluorescence of the CED-10::GFP::CED-10 marker than their younger, more distant siblings. The signal was not detectable until the 50-cell stage of the gastrulating embryo, when the marker started to accumulate beneath the cell boundaries. The signal gradually strengthened until the 3-fold stage, and remained unchanged thereafter until adulthood. The marker was expressed ubiquitously in all cells except the germ line Z2 and Z3 cells.
• Primary Identifier: Expr4493
• Subcellular Localization: At the subcellular level, the signal was absent in the nucleus, easily identified as a circular void within the cell. The distribution of the marker in cytosol sometimes assumed a vesicular structure. The intracellular vesicles in the scanned images overlapped with most, if not all, the granules observed by differential interference contrast microscopy. The marker accumulated at the boundaries between neighboring cells, appearing much more sparsely at cell surfaces facing the egg shell where no neighboring cell was present, and little accumulation was seen at the boundary facing the germ line cells Z2 and Z3. Large round vesicular structures exhibiting the strongest signals correspond to the cells undergoing apoptosis, indicating the cell boundaries between cells undergoing programmed cell death and the cells engulfing the dying cells.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a cellular object that consists of subcellular components, expresses genes or functions.	Cell	Cell type	WBbt:0004017

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000424	ced-10	C09G12.8	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010