WormMine: ExpressionPattern Expr838

• Pattern: In embryos, PEB-1 protein was strongly detected in the nuclei of many cells in the developing pharynx. Staining was first detected at the comma stage. Some pharyngeal cells do not express PEB-1, observed a cluster of nuclei that do not contain PEB-1 near the center of the developing pharynx, a region containing many of the pharyngeal neurons. Strong pharyngeal staining persisted through the remainder of embryogenesis. PEB-1 staining was also observed outside the pharynx in several cells near the rectum and in the tail and at lower level in hypodermal nuclei. In early larvae, PEB-1 protein is easily detectable in the pharynx, as well as in a subset of nonpharyngeal. Consistently observed PEB-1 protein in the nuclei of all pharyngeal muscles, marginal cells, epithelial cells, gland cells, and pharyngeal-intestinal valve cells. Notably, authors did not observe PEB-1 staining in any pharyngeal neurons. Pharyngeal PEB-1 staining decreased in late larvae until it became undetectable in adults. PEB-1 protein expression was also observed in some tissues outside the pharynx in larvae and adults. In general, the nonpharyngeal expression was weaker than expression in the pharynx, and it appeared dynamic during the worm life cycle. In larvae, PEB-1 antibody staining was reproducibly observed in several cells near the rectum and in hypodermal nuclei in the tail and in the developing vulva and surrounding cells. Authors also observed protein expression in several nuclei in the head and tail that were provisionally identified as hypodermal nuclei. In older larvae and adults, the rectal and vulval expression decreased, but PEB-1 staining was visible in germ-line cells. This expression is consistent with Northern blots indicating that peb-1 mRNA is expressed in the germ line.
• Primary Identifier: Expr838
• Remark: See Expr837, 839, 840 for Expr_pattern of the same locus.
• Subcellular Localization: PEB-1 protein was strongly detected in the nuclei.

12 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
A group of six equivalent cells forms a tightly constructed `valve` that links the posterior bulb of the pharynx to the anterior four cells of the intestine. These six cells comprise a small epithelial channel with a cuticular lining in continuity with the pharyngeal cuticle and link the lumen of the pharynx to the large lumen of the anterior intestine.	pharyngeal-intestinal valve	cardia	WBbt:0005767
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
Secretory gland cell of the pharynx	Pharyngeal gland cell		WBbt:0005788
cell that makes up epithelium in pharynx.	pharyngeal epithelial cell	pharyngeal epithelium	WBbt:0005459
Tail ventral hypodermis	hyp10	lineage name: ABplppppppp	WBbt:0004378
Tail ventral hypodermis, 8th hypodermis cell	hyp8		WBbt:0004381
Tail ventral hypodermis, 9th hypodermis cell	hyp9		WBbt:0004380
Tail dorsal hypodermis	hyp11	lineage name: Cpappv	WBbt:0004377
epithelium connecting intestine and anus.	rectal epithelium		WBbt:0005800
a type of pharyngeal cell that connects basement membrane to the apices of triangle-shaped pharyngeal lumen.	marginal cell		WBbt:0003673

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003968	peb-1	T14F9.4	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002