WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

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Expression Pattern :

Pattern  At the late first larval (L1) stage, INX-3 is present transiently in some newly generated cells. The postembryonic motor neurons, descendants of the Pn.a cells, express INX-3 briefly. INX-3 is also detected briefly in cells of the first two divisions of the M blast cell, coelomocytes, and sex muscles. By the comma stage, corresponding to early embryonic morphogenesis, INX-3 is still broadly expressed, but the pattern of expression becomes more restricted as morphogenesis proceeds. Because INX-3 is localized principally in puncta at plasma membranes, it is hard to assign expression unambiguously to individual cells; however, expression in major cell types or organs is clear. Double-labeling embryos with anti-INX-3 and MH27, a mAb that binds AJM-1 in apical epithelial intercellular junctions, indicated that, at the comma stage, INX-3 is localized to the developing intestine, pharynx, and hypodermis (epidermis), at minimum. During late morphogenesis, from the 3-fold stage until hatching, INX-3 is found principally in the posterior pharynx (isthmus and terminal bulb), at the anteriormost tip of the pharynx, in the region of the posterior intestine (probably intestinal muscles or rectal cells) and in the hypodermis. Expression in these tissues continues throughout development into adulthood with the exception of the hypodermis. Hypodermal expression is strong at the time of hatching, and INX-3 is present in plaques at the intercellular boundaries between most hypodermal cells except at the ventral midline between paired P cells; however, INX-3 becomes undetectable in the hypodermis shortly after hatching. INX-3 protein is first detected at the embryonic 2-cell stage. It is localized to small plaques at cellcell interfaces and can be detected throughout early embryogenesis in a pattern suggesting that most or all cells express inx-3. In adults, INX-3 is reduced such that only a few plaques are associated with vulval muscles. In the late L3 stage, INX-3 expression begins in the sex myoblasts (SMs). Expression continues in SM descendants so that all 16 sex muscles stain with anti-INX-3 in early L4 animals, confirming results obtained with an inx-3::gfp translational fusion gene. Primary Identifier  Expr2546
Remark  INX-3 detected during very early stages of development is likely to be maternally derived, since INX-3::GFP expressed zygotically is first detected by anti-GFP antibodies at approximately the 28-cell stage. Subcellular Localization  At embryonic 2-cell stage, localized to small plaques at cellcell interfaces. At the late first larval (L1) stage, INX-3 is present transiently in some newly generated cells, and in cells of the first two divisions of the M blast cell, coelomocytes, and sex muscles. INX-3 is readily detectable in the cytoplasm of these cells, as well as in cell-surface plaques. By the comma stage, INX-3 is localized principally in puncta at plasma membranes. At comma stage, within intestinal cells, whose large size allows easy visualization of subcellular location, INX-3 is localized to the basal portion of lateral membranes.

67 Anatomy Terms

Definition Name Synonym Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages. intestine gut WBbt:0005772
Epidermal layer. hypodermis epidermis WBbt:0005733
muscle associated with hermaphrodite vulva. vulval muscle   WBbt:0005821
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine. pharynx esophagus WBbt:0003681
neuron making synapse on an effector (muscle, gland). motor neuron motoneuron WBbt:0005409
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles. coelomocyte   WBbt:0005751
muscle lining of the uterine wall. uterine muscle   WBbt:0005342
embryonic cell MSa   WBbt:0006086
embryonic cell MSp   WBbt:0005912
Embryonic founder cell MS lineage name: P0.paa WBbt:0004458
embryonic cell ABalp   WBbt:0005865
Embryonic founder cell E lineage name: P0.pap WBbt:0004804
embryonic cell ABarp   WBbt:0006653
embryonic cell ABprp   WBbt:0006451
embryonic cell Ep   WBbt:0006612
embryonic cell ABplp   WBbt:0006279
embryonic cell ABarpp   WBbt:0006046
embryonic cell ABalpp   WBbt:0006486
embryonic cell MSpa   WBbt:0006142
embryonic founder cell Psub3 lineage name: P0.ppp WBbt:0006875
embryonic cell Cpa   WBbt:0006281
embryonic founder cell Psub1 lineage name: P0.p WBbt:0006874
embryonic founder cell Psub2 lineage name: P0.pp WBbt:0006873
Embryonic founder cell D lineage name: P0.pppa WBbt:0004873
embryonic cell MSpp   WBbt:0006255
Embryonic founder cell of germ line Psub4 lineage name: P0.pppp WBbt:0004421
embryonic cell EMS   WBbt:0006876
embryonic cell ABplap   WBbt:0006096
embryonic cell ABalap   WBbt:0006101
embryonic cell ABala   WBbt:0006707

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00002125 inx-3 F22F4.2 Caenorhabditis elegans

6 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. embryo Ce WBls:0000003