WormMine: ExpressionPattern Expr15986

• Pattern: We found that DAF-4::GFP::3xFLAG is expressed in multiple tissues, including the pharynx, hypodermis, intestine, and developing vulva. Within each cell, DAF-4::GFP::3xFLAG is primarily localized to the cell surface. We also detected some GFP signal in intracellular vesicles in hypodermal cells. Notably, in both the intestine and the vulva, 2 types of polarized epithelial cells, DAF-4::GFP::3xFLAG is localized to the basolateral membrane but absent on the apical membrane. The basolateral localization of DAF-4::GFP::3xFLAG in the intestinal cells has also been previously reported by Gleason and colleagues using a functional, intestinally expressed DAF-4::GFP.
• Primary Identifier: Expr15986

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
female genital.	vulva		WBbt:0006748
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000900	daf-4	C05D2.1	Caenorhabditis elegans

0 Life Stages