WormMine: ExpressionPattern Expr13253

• Pattern: Adult males exhibit daf-7 expression in both the ASI and the ASJ neuron pairs in the absence of P. aeruginosa. This expression pattern difference in adult C. elegans, can be observed both through the use of a pdaf-7::gfp transcriptional reporter as well as by fluorescence in situ hybridization (FISH) that directly probes for endogenous daf-7 mRNA. In populations of early larval animals carrying the pdaf-7::gfp transcriptional reporter, males and hermaphrodites were indistinguishable, with fluorescence observed only in the ASI neurons. To determine the onset of daf-7 expression in males, we imaged animals carrying the fluorescent reporter at multiple time points during development beginning in the L4 larval stage and extending into reproductive maturity. We found that daf-7 expression remains off in the ASJ of males through the end of the last (L4) larval stage, but is switched on during young adulthood as the animals reach reproductive maturity. In addition, we examined expression of daf-7 in the ASI neuron pair at several matched timepoints during development and observed a significant drop in the expression of daf-7 in the ASI neurons from L4 to young adulthood (52 hr and 64.5 hr timepoints). At subsequent timepoints during adulthood, however, expression levels in the ASI neurons remained constant as expression in the ASJ neurons increased (64.5 hr and 71.5 hr timepoints).
• Primary Identifier: Expr13253

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASIR	lineage name: ABpraapapppa	WBbt:0003887
one member of amphid neurons, single ciliated ending, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASIL	lineage name: ABplaapapppa	WBbt:0003888
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJL	lineage name: ABalpppppppa	WBbt:0003886
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASJR	lineage name: ABpraaappppa	WBbt:0003885
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000903	daf-7	B0412.2	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The stage after an male animal is fully-developed and reaches maturity.		adult male Ce	WBls:0000056