WormMine: ExpressionPattern Expr2269

• Pattern: Wild-type adults of either sex expressed both GLD-3L and GLD-3S. By contrast, adult glp-1(ts) hermaphrodites, which have virtually no germline, had no detectable GLD-3, consistent with the idea that gld-3 is expressed specifically in the germline. Furthermore, the deletion mutant, gld-3(q730), made no detectable GLD-3, confirming specificity of the antibodies.
• Primary Identifier: Expr2269
• Subcellular Localization: The GLD-3 subcellular distribution was investigated by immunocytochemistry. Similar results were obtained using either anti-GLD-3 or anti-GLD-3L. In most experiments, antibodies to the P granule protein PGL-1 were used to highlight germ cells and P granules. The data support three main conclusions. First, GLD-3 protein is predominantly cytoplasmic. GLD-3 was found in germline cytoplasm throughout development in both sexes and in the cytoplasm of early embryos. Second, GLD-3 distribution is controlled spatially and temporally. In adult hermaphrodites, GLD-3 was detectable in the transition zone (TZ), where germ cells enter meiotic prophase; it was fainter in the early mitotic region and in pachytene germ cells; however, as germ cells entered diakinesis, GLD-3 became abundant. During spermatogenesis, GLD-3 was present in primary spermatocytes, but not in secondary spermatocytes or mature sperm. A similar pattern was observed in males. Third, GLD-3 colocalizes with P granules. GLD-3 was found in the cytoplasm of early embryos, and colocalized with P granules from the 1-cell stage through the 64-cell stage; beyond the 100-cell stage of embryogenesis, GLD-3 was no longer detected. GLD-3 also appeared in particles that were near but not coincident with P granules. In the germline, GLD-3 was found both diffusely as well as in a more granular form. The granular GLD-3 was found primarily at the cytoplasmic side of the nuclear boundary and often colocalized with PGL-1. Mutant embryos derived from gld-3(q730) homozygous mothers could contain apparently normal P granules that appeared to segregate normally to a single blastomere; however, these gld-3 mutant embryos had no detectable GLD-3, consistent with the idea that this mutant removes most gld-3 activity.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001597	gld-3	T07F8.3	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	The C. elegans life stage spanning 0-100min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 28 cells. From first cleavage until the start of gastrulation.		blastula embryo Ce	WBls:0000005
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057