WormMine: ExpressionPattern Expr2456

• Pattern: COL-19::GFP expression was also examined in dauer stage larvae. After allowing TP12:kaIs(col-19::gfp) strain to starve, a large population of dauer larvae were obtained, examined, and found not to express the COL-19::GFP protein in their cuticles. Likewise, no annuli or ala-specific GFP expression was detected in temperatureinduced dauers of a daf-2(e1370) mutant allele strain crossed with TP12:kaIs(col-19::gfp) males. COL-19::GFP is expressed exclusively in the adult cuticle, with a distinctive spatial expression pattern. The tagged protein is expressed in both forms of hypodermally derived cuticle, specifically, the matrix of the circumferential transverse annuli and the tri-laminate lateral alae. This dominant cuticle matrix expression was similar between adult hermaphrodites and males. In addition, the hermaphrodite-specific vulva fluoresced strongly, as did the male-specific tail structures, including the cuticular ray and fan structures.
• Primary Identifier: Expr2456
• Remark: The widespread adult cuticle expression pattern is remarkably similar to that previously described for rabbit polyclonal antibodies raised against total adult cuticles and probed against the surface of live worms, supporting the prediction that the GFP-tagged COL-19 expression may reflect the endogenous COL-19 expression pattern. Additionally, a recent study analyzing the localization of a Ty-tagged DPY-13 transgene displayed a remarkably similar annuli expression pattern to that described here for COL-19::GFP.
• Subcellular Localization: COL-19::GFP was expressed in a transverse banding pattern of approximately 1.2 um in thickness, consistent with the annuli, and was absent from the annular furrows.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
a rigid external coating which is secreted by the hypodermis, seam and some transitional epithelial cells which covers the outer body, the major openings into the body cavity from the exterior, and two large sensory bristles, the male spicules. The cuticle of the spicules, the hook, and of some portions of the spicule channels is especially rigid and is probably sclerotized. Similar sclerotic features are occasionally noted in the ventral surface of the male tail fan.	cuticle	exoskeleton	WBbt:0005755
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000608	col-19	ZK1193.1	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A third stage larva specialized for dispersal and long term survival.		dauer larva Ce	WBls:0000032
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057