WormMine: ExpressionPattern Expr3261

• Pattern: The col-43::gfp fusion gene pC3894G was expressed in all hypodermal cells in the L2d stage. col-43 transcripts in the L2d stage were also confirmed by in situ hybridization. The L2d and dauer larvae develop to the adult worm through the L3 and L4 larval stages, respectively, after the environment shifts to nutrient-rich conditions. pC3894G was continuously expressed in L3; however, it was rarely expressed in L4 and adults recovered from L2d larvae. The expression of the col-43::gfp fusion gene pCTL3894G was not detected due to the signal peptide produced by this fusion gene. The COL-43::GFP fusion protein could be transported into the extracellular matrix and diffuse to an undetectable level. Two promoter deletion constructs, pC1345G and pC1292R, were expressed in the hypodermal cells in the L2d and the L3 stage recovered from the L2d stage. These promoter deletion constructs were continuously expressed in the dauer, the L4 and the adult recovered from stage L2. The additional promoter deletion construct, pC610G was also expressed in the hypodermal cells in the L2d and recovered L3 stage, but was rarely expressed in the L4 stage and adult recovered from the L2d stage. These results indicate that two upstream regions of col-43, within 0.6 kb and from 0.6 kb to 1.3 kb, were required for the expression and up-regulation in the dauer cycle of col-43 expressions, respectively. In the late adult stage the ectopic expressions of three promoter deletion constructs in the uterus and posterior intestinal cells were considered to be endogenous misexpressions of a series of pPD vectors.
• Primary Identifier: Expr3261

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000620	col-43	ZC513.8	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	A special second stage larva with altered physiology and developmental potential that leads to formation of dauer larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2d larva Ce	WBls:0000046