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Expr1872
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In late L2 larvae anti-PAG-3 staining was seen in approximately two dozen cells in the head, all six mechanosensory neurons, the BDU neurons, approximately ten cells in the tail as well as in the ventral cord. PAG-3 staining of many cells in the head and tail remained detectable in adult animals. In the ventral cord, PAG-3 was first detected in the Pn.aa neuroblasts. PAG-3 was not detected in the Pn.ap euroblasts or their descendants. PAG-3 was present equally in each of the descendant cells of Pn.aa after subsequent rounds of division (i.e. the Pn.aaa, Pn.aap, Pn.aaaa and Pn.aaap cells), including the three differentiating neurons generated by each Pn.aa neuroblast. In most cells, PAG-3 protein became undetectable shortly after the cells had been generated in the L1, but PAG-3 was present in six cells in the ventral cords of adults. In late L2 larvae anti-PAG-3 staining was seen in approximately two dozen cells in the head, all six mechanosensory neurons, the BDU neurons, approximately ten cells in the tail as well as in the ventral cord. PAG-3 staining of many cells in the head and tail remained detectable in adult animals. In the ventral cord, PAG-3 was first detected in the Pn.aa neuroblasts. PAG-3 was not detected in the Pn.ap neuroblasts or their descendants. PAG-3 was present equally in each of the descendant cells of Pn.aa after subsequent rounds of division (i.e. the Pn.aaa, Pn.aap, Pn.aaaa and Pn.aaap cells), including the three differentiating neurons generated by each Pn.aa neuroblast. In most cells, PAG-3 protein became undetectable shortly after the cells had been generated in the L1, but PAG-3 was present in six cells in the ventral cords of adults. PAG-3 expression persisted throughout the life of the animal in four cells in the retrovesicular ganglion at the anterior end of the ventral cord and in two cells in the posterior ventral cord. In newly hatched L1-stage larvae, before the initiation of the postembryonic W and P cell lineages, two cells in the retrovesicular ganglion expressed PAG-3. Based on position, these cells were most likely the RIG interneurons. After completion of the W and P cell lineages, two additional cells in the retrovesicular ganglion and two cells in the posterior ventral cord contained detectable PAG-3 protein. These nuclei might be the two AVF and the VA11 and VA12 neurons, respectively. This hypothesis was confirmed by staining animals carrying an integrated Punc-4lacZ reporter, which is expressed in the AVF and VA as well as other neurons, with PAG-3 antiserum and monoclonal antibody against beta-galactosidase. PAG-3 protein was expressed more widely in the nervous system than had been observed using the Ppag-3lacZ reporter. PAG-3 was detected during embryonic development in many nuclei ~280 minutes after fertilization. |
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Transgenic Marker: rol-6(su1006). |
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Expr521
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Expression in touch-sensitive neurons, motor neurons, and interneurons which starts in the 2-fold embryo and disappears by L3. Sporadic expression seen in retrovesicular ganglion at L1. Expressed in ALM, PLM and BDU cells at 2-fold embryo, L1 and L2. In AVM, PVM cells at L1 and L2. Expressed in ventral cord motor neurons VA2-VA12, VB1-VB11, AVF cells, and precursors at L1 and L2. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr668
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Postembryonic expression is observed in the rectum epithelium. A major site of EGL-5 expression is in the rectal epithelium. At hatching, the rectal expression is in K, F, B, U and Y. In addition expression is seen in (Y differentiates into) PDA motor neuron, (K divides to rise to) part of dorsal rectal epithelium and a cell that becomes DVB motor neuron. In males male-specific neurons show expression. In males Ab staining is observed in B.a and B.p as well as Y.p and Y.p in L1 and early L2. It appears that most/all B, Y, U, F, K descendants express EGL-5. Ventral neuroblast P12, staining is first seen in P12.a and P12.p in 12-h worms. Staining is maintained in P12 descendants in 15-h until adulthood. Both sexes' mechanosensory neurons, expression is seen in PLM neurons throughout larval development. In addition two cells express EGL-5, one in anterior region of each lumbar ganglion, likely to be PVC interneurons. Both sexes' muscles cells, expression is detected in 4-6 left/right pairs of posterior body-wall muscle cells in L1 larvae at earliest examined time 10-12 h. At L2 staining is detected in 12 left/right pairs of nuclei. Staining is strongest in the most posterior nuclei and tapers of towards the anterior. Staining in posterior body wall muscle cells remains throughout larval development and into adulthood in both sexes. In L3 males, sex-specific muscle lineages and sex-specific muscles stain strongly. These muscles include the diagonal muscles, muscles of spicule, gubernaculum and other sex muscles. Staining in these muscles persist until adulthood. HSN neurons, expression from L1 onwards through to adulthood. Male gonad, first detected in the male gonad in late L1 in a group of 6 cells at the anterior end. It appears that expression is clustered in a region that consists of both somatic cells and germ cells. Later at the beginning of the late mitotic period, staining nuclei lose their clustered arrangement. By 34 h, staining is seen in several dividing cells that form the primordium of the seminal vesicles as well as in two large nuclei in the valve region. In the nuclei of diving cells staining surrounds a condensed chromatin. This pattern persists until the end of the late mitotic period (35-37 h posthatching) when staining is also detected in sperm cells. No staining was observed in cells of the vas deferens. Lateral hypodermis, expression is seen in male seam from mid-L2. Staining first appears in V6.ppp at 20-22 h postembryonic development. Staining persists in V6.pppa and V6.pppp but at a lower level. Intensity of staining increases in R5 and R6 and to lesser degree in R4. Identification of staining cells in ray sublineages was not possible due to intense fluorescence of B-lineage cells lying in the same region. However it was possible to observe expression of a reporter gene in R4, R5, and R6 and also in cells of the R5 and R6 sublineages. |
Expressed in the nuclei. |
