WormMine: ExpressionPattern Expr668

• Pattern: Postembryonic expression is observed in the rectum epithelium. A major site of EGL-5 expression is in the rectal epithelium. At hatching, the rectal expression is in K, F, B, U and Y. In addition expression is seen in (Y differentiates into) PDA motor neuron, (K divides to rise to) part of dorsal rectal epithelium and a cell that becomes DVB motor neuron. In males male-specific neurons show expression. In males Ab staining is observed in B.a and B.p as well as Y.p and Y.p in L1 and early L2. It appears that most/all B, Y, U, F, K descendants express EGL-5. Ventral neuroblast P12, staining is first seen in P12.a and P12.p in 12-h worms. Staining is maintained in P12 descendants in 15-h until adulthood. Both sexes' mechanosensory neurons, expression is seen in PLM neurons throughout larval development. In addition two cells express EGL-5, one in anterior region of each lumbar ganglion, likely to be PVC interneurons. Both sexes' muscles cells, expression is detected in 4-6 left/right pairs of posterior body-wall muscle cells in L1 larvae at earliest examined time 10-12 h. At L2 staining is detected in 12 left/right pairs of nuclei. Staining is strongest in the most posterior nuclei and tapers of towards the anterior. Staining in posterior body wall muscle cells remains throughout larval development and into adulthood in both sexes. In L3 males, sex-specific muscle lineages and sex-specific muscles stain strongly. These muscles include the diagonal muscles, muscles of spicule, gubernaculum and other sex muscles. Staining in these muscles persist until adulthood. HSN neurons, expression from L1 onwards through to adulthood. Male gonad, first detected in the male gonad in late L1 in a group of 6 cells at the anterior end. It appears that expression is clustered in a region that consists of both somatic cells and germ cells. Later at the beginning of the late mitotic period, staining nuclei lose their clustered arrangement. By 34 h, staining is seen in several dividing cells that form the primordium of the seminal vesicles as well as in two large nuclei in the valve region. In the nuclei of diving cells staining surrounds a condensed chromatin. This pattern persists until the end of the late mitotic period (35-37 h posthatching) when staining is also detected in sperm cells. No staining was observed in cells of the vas deferens. Lateral hypodermis, expression is seen in male seam from mid-L2. Staining first appears in V6.ppp at 20-22 h postembryonic development. Staining persists in V6.pppa and V6.pppp but at a lower level. Intensity of staining increases in R5 and R6 and to lesser degree in R4. Identification of staining cells in ray sublineages was not possible due to intense fluorescence of B-lineage cells lying in the same region. However it was possible to observe expression of a reporter gene in R4, R5, and R6 and also in cells of the R5 and R6 sublineages.
• Primary Identifier: Expr668
• Remark: This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).
• Subcellular Localization: Expressed in the nuclei.

45 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
neurons that sense body touch, have specialized microtubules in processes.	touch receptor neuron	microtubule cell	WBbt:0005237
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
muscle lining of the uterine wall.	uterine muscle		WBbt:0005342
descendent cell of P12, posterior	P12.p		WBbt:0006900
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCL	lineage name: ABplpppaapaa	WBbt:0004094
Ventral cord interneuron, cell body in lumbar ganglion, synapses onto VB and DB motor neurons, formerly called delta.	PVCR	lineage name: ABprpppaapaa	WBbt:0004092
Male haploid germ cell.	sperm	spermatozoa	WBbt:0006798
	spicule retractor muscle		WBbt:0005827
	spicule protractor muscle		WBbt:0005826
Neuron class of one ring interneuron, cell body in dorsorectal ganglion, inervates rectal muscles.	DVB	lineage name: K.p	WBbt:0004822
epithelium connecting intestine and anus.	rectal epithelium		WBbt:0005800
cell, posterior daughter of B cell in male	B.p		WBbt:0006986
cell, anterior daughter of B cell in male	B.a		WBbt:0006985
Rectal cell, postembryonic blast cell in male	B cell	lineage name: ABprppppapa	WBbt:0003825
Rectal cell, blast cell in male	F cell	lineage name: ABplppppapp	WBbt:0004799
descendent cell of P12, anterior	P12.a		WBbt:0006888
specialized muscle in the male.	diagonal muscle	dgl	WBbt:0005815
specialized muscle type in the posterior of adult male.	longitudinal male muscle	mu_male_long	WBbt:0006909
Neuron class of one motor neuron, process in dorsal cord, same as Y cell in hermaphrodite, Y.a in male.	PDA		WBbt:0004386
Rectal cell, postembryonic blast cell in male	U cell	lineage name: ABplppppapa	WBbt:0004942
Rectal cell, postembryonic blast cell	K cell	lineage name: ABplpapppaa	WBbt:0004499
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850
	gubernacular erector muscle		WBbt:0005824
specialized muscle in the male.	posterior oblique muscle		WBbt:0005823
specialized muscle in the male.	anterior oblique muscle		WBbt:0005822
	gubernacular retractor muscle		WBbt:0005825
Rectal cell at hatching, becomes PDA in hermaphrodite, postembryonic blast cell in male	Y cell	lineage name: ABprpppaaaa	WBbt:0004578

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001174	egl-5	C08C3.1	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003