WormMine: ExpressionPattern Expr1872

• Pattern: In late L2 larvae anti-PAG-3 staining was seen in approximately two dozen cells in the head, all six mechanosensory neurons, the BDU neurons, approximately ten cells in the tail as well as in the ventral cord. PAG-3 staining of many cells in the head and tail remained detectable in adult animals. In the ventral cord, PAG-3 was first detected in the Pn.aa neuroblasts. PAG-3 was not detected in the Pn.ap euroblasts or their descendants. PAG-3 was present equally in each of the descendant cells of Pn.aa after subsequent rounds of division (i.e. the Pn.aaa, Pn.aap, Pn.aaaa and Pn.aaap cells), including the three differentiating neurons generated by each Pn.aa neuroblast. In most cells, PAG-3 protein became undetectable shortly after the cells had been generated in the L1, but PAG-3 was present in six cells in the ventral cords of adults. In late L2 larvae anti-PAG-3 staining was seen in approximately two dozen cells in the head, all six mechanosensory neurons, the BDU neurons, approximately ten cells in the tail as well as in the ventral cord. PAG-3 staining of many cells in the head and tail remained detectable in adult animals. In the ventral cord, PAG-3 was first detected in the Pn.aa neuroblasts. PAG-3 was not detected in the Pn.ap neuroblasts or their descendants. PAG-3 was present equally in each of the descendant cells of Pn.aa after subsequent rounds of division (i.e. the Pn.aaa, Pn.aap, Pn.aaaa and Pn.aaap cells), including the three differentiating neurons generated by each Pn.aa neuroblast. In most cells, PAG-3 protein became undetectable shortly after the cells had been generated in the L1, but PAG-3 was present in six cells in the ventral cords of adults. PAG-3 expression persisted throughout the life of the animal in four cells in the retrovesicular ganglion at the anterior end of the ventral cord and in two cells in the posterior ventral cord. In newly hatched L1-stage larvae, before the initiation of the postembryonic W and P cell lineages, two cells in the retrovesicular ganglion expressed PAG-3. Based on position, these cells were most likely the RIG interneurons. After completion of the W and P cell lineages, two additional cells in the retrovesicular ganglion and two cells in the posterior ventral cord contained detectable PAG-3 protein. These nuclei might be the two AVF and the VA11 and VA12 neurons, respectively. This hypothesis was confirmed by staining animals carrying an integrated Punc-4lacZ reporter, which is expressed in the AVF and VA as well as other neurons, with PAG-3 antiserum and monoclonal antibody against beta-galactosidase. PAG-3 protein was expressed more widely in the nervous system than had been observed using the Ppag-3lacZ reporter. PAG-3 was detected during embryonic development in many nuclei ~280 minutes after fertilization.
• Primary Identifier: Expr1872

66 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neurons that sense body touch, have specialized microtubules in processes.	touch receptor neuron	microtubule cell	WBbt:0005237
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC4	lineage name: P6.aap	WBbt:0004617
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC5	lineage name: P7.aap	WBbt:0004613
Ventral cord motor neuron, innervates ventral body muscles	VB9	lineage name: P8.aaap	WBbt:0004627
Ventral cord motor neuron, innervates ventral body muscles	VB10	lineage name: P9.aaap	WBbt:0004625
Ventral cord motor neuron, innervates ventral body muscles	VB11	lineage name: P10.aaap	WBbt:0004623
Ventral cord motor neuron, innervates ventral body muscles	VB5	lineage name: P4.aaap	WBbt:0004637
Ventral cord motor neuron, innervates ventral body muscles	VB6	lineage name: P5.aaap	WBbt:0004633
Ventral cord motor neuron, innervates ventral body muscles	VB7	lineage name: P6.aaap	WBbt:0004631
Ventral cord motor neuron, innervates ventral body muscles	VB8	lineage name: P7.aaap	WBbt:0004629
Ventral cord motor neuron, innervates ventral body muscles, also interneuron in ring	VB1	lineage name: P1.aaap	WBbt:0004643
Ventral cord motor neuron, innervates ventral body muscles	VB3	lineage name: P2.aaap	WBbt:0004639
Ventral cord motor neuron, innervates ventral body muscles	VB4	lineage name: P3.aaap	WBbt:0004638
Ganglion that lies at the anterior limit of the ventral nerve cord, near the ventral ganglion and nerve ring in the head posterior to the excretory pore. It is open and continuous with the region containing the motoneurons of the ventral cord. In the early L1 this ganglion holds 12 neuron cell bodies plus one neuroblast (Sulston and Horvitz, 1977; White et al., 1986). In the adult animal, the ganglion holds 20 neuron cell bodies.	retrovesicular ganglion		WBbt:0005656
Neuron, process runs along excretory canal and into ring, unique darkly staining synaptic vesicles	BDUL	lineage name: ABarppaappp	WBbt:0003812
Neuron, process runs along excretory canal and into ring, unique darkly staining synaptic vesicles	BDUR	lineage name: ABarpppappp	WBbt:0003811
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC6	lineage name: P8.aap	WBbt:0004611
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC1	lineage name: P3.aap	WBbt:0004621
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC2	lineage name: P4.aap	WBbt:0004619
Hermaphrodite specific ventral cord motor neuron innervates vulval muscles and ventral body muscles	VC3	lineage name: P5.aap	WBbt:0004618
Ventral cord motor neuron, innervates ventral body muscles, but also interneuron in preanal ganglion	VA12	lineage name: P12.aaaa	WBbt:0004645
Ring interneuron	RIGR	lineage name: ABprppappaa	WBbt:0003930
Ring interneuron	RIGL	lineage name: ABplppappaa	WBbt:0003932
Interneuron, processes in ventral cord and ring, few synapses, right side	AVFR		WBbt:0005658
Interneuron, processes in ventral cord and ring, few synapses, left side	AVFL		WBbt:0005657
Ventral cord motor neuron, innervates ventral body muscles	VA2	lineage name: P2.aaaa	WBbt:0004870
Ventral cord motor neuron, innervates ventral body muscles	VA11	lineage name: P11.aaaa	WBbt:0004647
Ventral cord motor neuron, innervates ventral body muscles	VA10	lineage name: P10.aaaa	WBbt:0004649
Ventral cord motor neuron, innervates ventral body muscles	VA8	lineage name: P8.aaaa	WBbt:0004653
Ventral cord motor neuron, innervates ventral body muscles	VA9	lineage name: P9.aaaa	WBbt:0004651

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003909	pag-3	F45B8.4	Caenorhabditis elegans

8 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013