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Expr1872
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In late L2 larvae anti-PAG-3 staining was seen in approximately two dozen cells in the head, all six mechanosensory neurons, the BDU neurons, approximately ten cells in the tail as well as in the ventral cord. PAG-3 staining of many cells in the head and tail remained detectable in adult animals. In the ventral cord, PAG-3 was first detected in the Pn.aa neuroblasts. PAG-3 was not detected in the Pn.ap euroblasts or their descendants. PAG-3 was present equally in each of the descendant cells of Pn.aa after subsequent rounds of division (i.e. the Pn.aaa, Pn.aap, Pn.aaaa and Pn.aaap cells), including the three differentiating neurons generated by each Pn.aa neuroblast. In most cells, PAG-3 protein became undetectable shortly after the cells had been generated in the L1, but PAG-3 was present in six cells in the ventral cords of adults. In late L2 larvae anti-PAG-3 staining was seen in approximately two dozen cells in the head, all six mechanosensory neurons, the BDU neurons, approximately ten cells in the tail as well as in the ventral cord. PAG-3 staining of many cells in the head and tail remained detectable in adult animals. In the ventral cord, PAG-3 was first detected in the Pn.aa neuroblasts. PAG-3 was not detected in the Pn.ap neuroblasts or their descendants. PAG-3 was present equally in each of the descendant cells of Pn.aa after subsequent rounds of division (i.e. the Pn.aaa, Pn.aap, Pn.aaaa and Pn.aaap cells), including the three differentiating neurons generated by each Pn.aa neuroblast. In most cells, PAG-3 protein became undetectable shortly after the cells had been generated in the L1, but PAG-3 was present in six cells in the ventral cords of adults. PAG-3 expression persisted throughout the life of the animal in four cells in the retrovesicular ganglion at the anterior end of the ventral cord and in two cells in the posterior ventral cord. In newly hatched L1-stage larvae, before the initiation of the postembryonic W and P cell lineages, two cells in the retrovesicular ganglion expressed PAG-3. Based on position, these cells were most likely the RIG interneurons. After completion of the W and P cell lineages, two additional cells in the retrovesicular ganglion and two cells in the posterior ventral cord contained detectable PAG-3 protein. These nuclei might be the two AVF and the VA11 and VA12 neurons, respectively. This hypothesis was confirmed by staining animals carrying an integrated Punc-4lacZ reporter, which is expressed in the AVF and VA as well as other neurons, with PAG-3 antiserum and monoclonal antibody against beta-galactosidase. PAG-3 protein was expressed more widely in the nervous system than had been observed using the Ppag-3lacZ reporter. PAG-3 was detected during embryonic development in many nuclei ~280 minutes after fertilization. |
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Transgenic Marker: rol-6(su1006). |
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Expr521
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Expression in touch-sensitive neurons, motor neurons, and interneurons which starts in the 2-fold embryo and disappears by L3. Sporadic expression seen in retrovesicular ganglion at L1. Expressed in ALM, PLM and BDU cells at 2-fold embryo, L1 and L2. In AVM, PVM cells at L1 and L2. Expressed in ventral cord motor neurons VA2-VA12, VB1-VB11, AVF cells, and precursors at L1 and L2. |
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Expr1516
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About 8 hour after hatching, P cells descend into the ventral nerve cord and interdigitate to form a single row of cells of P1-P12. In both sexes, mab-5 was detected in P7-P10. In older larva, mab-5 was detected in P7-P12, but not more anterior cells. In hermaphrodites, expression pattern was similar to males with two exceptions. The p(9-11).app cells and p(9-11).p cells no longer express MAB-5 by L2. In male, descendants of P10 consistently express high levels of MAB-5, those of P9 and P11 express low levels, and those of P7 and P8 express barely detectable levels. P12 descendants express MAB-5 only until the division of P12.a. The descendants of P cells express comparable levels of MAB-5 with exceptions to Pn.p cells, they express lower levels than their neighbors. Expression levels in P descendants were similar to those seen in the neighboring juvenile motoneurons. Both sets of cells expressed MAB-5 in a graded pattern, tapering off toward the anterior. In newly hatched larva, in both sexes, mab-5 was detected consistently in nuclei of P9/10 and P11/12, but not more anterior P cells. mab-5 was also detected at high levels in the posterior juvenile motoneurons, located ventrally. MAB-5 is localized to the posterior of the body, including P7-P12 in the P lineage but not in more anterior cells. Within P7-P12, MAB-5 expression was graded with the highest levels in the P10 descendants. The descendants of any one P cell expressed comparable levels of MAB-5. |
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