WormMine: LifeStage WBls:0000687

• Definition: The fifth sub-stage of the L4 larva. utse is visible as a thin layer separating the vulval and uterine lumens. 'Fingers' are formed at the sides of the vulva next to vulB1 and vulB2. The uterine lumen is fully distended. At 20 Centigrade: 39.0 hours after hatching, 5.0 hours from the L3-to-L4 molt.
• Primary Identifier: WBls:0000687
• Public Name: L4.4 larva Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	A C. elegans life stage that occurs during the L4 larval life stage		C. elegans life stage occurring during L4 larval stage	WBls:0000821

0 Expression Clusters

14 Expression Patterns

• Primary Identifier: Expr16432
• Pattern: We detected DMD-9::GFP expression in 10 head neurons of hermaphrodites at the L4 stage. High dmd-9 expression was detected in the BAG, AFD, AWCon, AWCoff, ASEL, and AWB neurons, with lower expression in ASER, and the ASGs. We also observed weak and inconsistent expression in other head neurons and transient weak expression in the tail of hermaphrodites and males at the L4 stage. No overt differences were observed in DMD-9::GFP expression in neurons of hermaphrodites and males. We also detected DMD-9::GFP expression in non-neuronal tissues. Transient DMD-9::GFP expression was observed in the uterine cells at specific sub-stages of the L4 stage. DMD-9 is not expressed from L4.0 to L4.3, with expression first detected in the early L4.4 stage, with the highest level during L4.4. before gradual loss by the L4.9 stage (Mok et al. 2015). DMD-9::GFP was not detected in adult uterine cells. We also detected DMD-9::GFP expression in sperm of hermaphrodites and males. In addition, we found that in a small proportion of L4 animals DMD-9::GFP shows asymmetric expression in the ASEs and AWCs neurons.

• Primary Identifier: Expr16423
• Pattern: We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline.

• Primary Identifier: Expr12292
• Remark: To cite this work: Ghosh, Srimoyee; Inoue, Takao; Sternberg, Paul (2015): ina-1::gfp expression. WormBase. Dataset. DOI: http://dx.doi.org/10.17912/W2159J . Reviewed by Bhagwati Gupta. Funding: National Institutes of Health USPHS training grant GM07616.
• Pattern: ina-1 is expressed throughout uterine toroids. At mid L4, expression is seen in uterine toroid 1 and 4 (ut1, ut4) and in the anterior uterine toroids 2 and 3 (ut2, ut3). In addition, ina-1::GFP is detected in the spermathecal-uterine junction and spermatheca. At the L4 lethargus stage, bright expression is observed in uterine toroids 1 and 4, the spermathecal-uterine junction, and spermatheca. Dimmer expression is present in uterine toroid 2 and posterior toroid 3. Fluorescence is also detected in vulE.

• Primary Identifier: Expr15948
• Pattern: LPR-3 briefly marked all vulva cell apical surfaces at early L4.4, but then became restricted to 2 ̊ cells and then specifically to vulB1 and vulB2 before largely disappearing by L4.6-L4.7. The departure of LPR-3 from vulC and vulD coincided with the increasingly strong presence of NOAH-1 there.

• Primary Identifier: Expr12298

• Primary Identifier: Expr12304

• Primary Identifier: Expr12305
• Pattern: Only reporter in this study where expression was obviously turned off during L4.

• Primary Identifier: Expr15947
• Subcellular Localization: The transmembrane eLRRon protein LET-4 marked all vulval apical membranes during the late L4.2-L4.7 period, and thereafter appeared intracellular.

• Primary Identifier: Expr12301
• Pattern: Expression in E variable in L4.3and L4.4, consistent in later substages; expression in F variable throughout.

• Primary Identifier: Expr12300
• Pattern: Variable expression in all substages; low level of expression in B2, C from L4.1 to L4.7.

• Primary Identifier: Expr12299
• Pattern: Variable expression in L4.1; consistent in later sub-stages.

• Primary Identifier: Expr15949
• Pattern: NOAH-1 faintly marked all 2 ̊ vulva cell surfaces at L4.4-L4.5, but then became increasingly concentrated on vulC and vulD.

• Primary Identifier: Expr12306
• Pattern: Variable expression possibly due to loss of extrachromosomal array.

• Primary Identifier: Expr15946
• Pattern: The secreted eLRRon protein SYM-1 showed a limited pattern, labelling vulB1 and vulB2 for just a brief period at L4.4-L4.5.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The sixth sub-stage of the L4 larva. The side of the vulval lumen between vulC and vulD forms a smooth curve. At 20 Centigrade: 40.4 hours after hatching, 6.4 hours from the L3-to-L4 molt.		L4.5 larva Ce	WBls:0000688

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth sub-stage of the L4 larva. vulFs have separated and the apex of the lumen is flat and capped by the anchor cell. The uterine lumen starts to form. At 20 Centigrade: 38.1 hours after hatching, 4.1 hours from the L3-to-L4 molt.		L4.3 larva Ce	WBls:0000686

0 Sub Stages