WormMine: LifeStage WBls:0000692

• Definition: The tenth sub-stage of the L4 larva. The vulval lumen is completely collapsed. At 20 Centigrade: 42.8 hours after hatching, 8.8 hours from the L3-to-L4 molt.
• Primary Identifier: WBls:0000692
• Public Name: L4.9 larva Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	A C. elegans life stage that occurs during the L4 larval life stage		C. elegans life stage occurring during L4 larval stage	WBls:0000821

0 Expression Clusters

11 Expression Patterns

• Primary Identifier: Expr16432
• Pattern: We detected DMD-9::GFP expression in 10 head neurons of hermaphrodites at the L4 stage. High dmd-9 expression was detected in the BAG, AFD, AWCon, AWCoff, ASEL, and AWB neurons, with lower expression in ASER, and the ASGs. We also observed weak and inconsistent expression in other head neurons and transient weak expression in the tail of hermaphrodites and males at the L4 stage. No overt differences were observed in DMD-9::GFP expression in neurons of hermaphrodites and males. We also detected DMD-9::GFP expression in non-neuronal tissues. Transient DMD-9::GFP expression was observed in the uterine cells at specific sub-stages of the L4 stage. DMD-9 is not expressed from L4.0 to L4.3, with expression first detected in the early L4.4 stage, with the highest level during L4.4. before gradual loss by the L4.9 stage (Mok et al. 2015). DMD-9::GFP was not detected in adult uterine cells. We also detected DMD-9::GFP expression in sperm of hermaphrodites and males. In addition, we found that in a small proportion of L4 animals DMD-9::GFP shows asymmetric expression in the ASEs and AWCs neurons.

• Primary Identifier: Expr16423
• Pattern: We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline.

• Primary Identifier: Expr12298

• Primary Identifier: Expr12304

• Primary Identifier: Expr15945
• Pattern: MUP-4::GFP appeared at the L4.6-L4.7 stage, and was enriched over vulC and vulD, where the vm1 sex muscles attach. By L4.9 and adult, when cuticle is present, MUP-4::GFP lined all vulva apical membranes, but it was expressed most prominently in the vulA toroid, which links the vulva to the surrounding epidermis and body muscle.

• Primary Identifier: Expr15947
• Subcellular Localization: The transmembrane eLRRon protein LET-4 marked all vulval apical membranes during the late L4.2-L4.7 period, and thereafter appeared intracellular.

• Primary Identifier: Expr12301
• Pattern: Expression in E variable in L4.3and L4.4, consistent in later substages; expression in F variable throughout.

• Primary Identifier: Expr13790
• Pattern: strong ser-4b GFP expression was observed in VulF and VulE primary and VulD secondary epithelial cells. The ser-4b promoter also drove weak GFP expression in the vm2 muscles in L4.7-9, and this was elevated in adults.

• Primary Identifier: Expr12300
• Pattern: Variable expression in all substages; low level of expression in B2, C from L4.1 to L4.7.

• Primary Identifier: Expr12299
• Pattern: Variable expression in L4.1; consistent in later sub-stages.

• Primary Identifier: Expr12306
• Pattern: Variable expression possibly due to loss of extrachromosomal array.

0 Followed By

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The ninth sub-stage of the L4 larva. The vulval lumen is partially collapsed. At 20 Centigrade: 42.5 hours after hatching, 8.5 hours from the L3-to-L4 molt.		L4.8 larva Ce	WBls:0000691

0 Sub Stages