WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Life Stage :

Definition  At 20 Centigrade: 6-7 days after L4-adult molt. 9-10 days after first cleavage. Primary Identifier  WBls:0000670
Public Name  6-days post-L4 adult hermaphrodite Ce
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0 Anatomy Terms

2 Contained In

Remark Definition Other Name Public Name Primary Identifier
  The stage after an hermaphrodite animal is fully-developed and reaches maturity. adult hermaphrodite Ce WBls:0000057
  A C. elegans life stage that occurs during the adult life stage C. elegans life stage occurring during adulthood WBls:0000816

22 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
Dietary restriction Transcripts that showed significantly decreased expression after N2 animals were under dietary restriction (DR, OP50 OD = 0.1) from 3-day post L4 till 6-day post L4 adult hermaphrodite stage, comparing to under ad libtum (AL, OP50 OD = 3) condition. Bioconductor package edgeR, p < 0.05. WBPaper00056443:DietaryRestriction_downregulated
  Transcripts that showed significantly changed expression in 6-day post-L4 adult hermaphrodite comparing to in 1-day post L4 adult hermaphrodite animals. Sleuth WBPaper00051558:aging_regulated
Dietary restriction Transcripts that showed significantly increased expression after N2 animals were under dietary restriction (DR, OP50 OD = 0.1) from 3-day post L4 till 6-day post L4 adult hermaphrodite stage, comparing to under ad libtum (AL, OP50 OD = 3) condition. Bioconductor package edgeR, p < 0.05. WBPaper00056443:DietaryRestriction_upregulated
  Transcripts that showed significantly increased expression in daf-16(mu86);fer-15(b26ts) comparing to in fer-15(b26ts) at 6-day or 7-day post L4 adult hermaphrodite stage. DESeq2 (v1.18.1), ajusted p-value < 0.05 WBPaper00056218:daf-16(mu86)_upregulated_Day7
  Transcripts that showed significantly decreased expression in sek-1(km4) animals comparing to in N2 animals under both dietary (DR, OP50 OD = 0.1) and ad libtum (AL, OP50 OD = 3) conditions from 3-day post L4 till 6-day post L4 adult hermaphrodite stage. Bioconductor package edgeR, p < 0.05. WBPaper00056443:sek-1(km4)_downregulated
  Proteins that showed significantly increased expression in iff-1(RNAi) animals comparing to CF512 (fer-15(b26)II;fem-1(hc17)IV) animals applied with control vector at 15C. Two-tailed Student's t-test (n=3, P-value <0.05 was considered significant). WBPaper00057288:iff-1(RNAi)_upregulated_protein
  Transcripts that showed significantly decreased expression in the germline of iff-1(RNAi) animals comparing to the germine of N2 animals applied with control vector at 15C. Statistical comparisons were made by two-tailed t-test, n=3 (each replicate contains 150 extruded germ lines from N2 wild-type worms), P < 0.01 was considered significant. WBPaper00057288:iff-1(RNAi)_downregulated_transcript
  Transcripts that showed significantly increased expression in sek-1(km4) animals comparing to in N2 animals under both dietary (DR, OP50 OD = 0.1) and ad libtum (AL, OP50 OD = 3) conditions from 3-day post L4 till 6-day post L4 adult hermaphrodite stage. Bioconductor package edgeR, p < 0.05. WBPaper00056443:sek-1(km4)_upregulated
Temperature shift: 15C vs 20 C. Transcripts that showed significantly decreased expression in N2 animals treated with empty RNAi vector at 15C comparing to N2 animals treated with empty RNAi vector at 20C. Statistical comparisons were made by two-tailed t-test, n=3 (each replicate contains 150 extruded germ lines from N2 wild-type worms), P < 0.01 was considered significant. WBPaper00057288:Cold_downregulated_transcript
Temperature shift: 15C vs 20 C. Proteins that showed significantly decreased expression in CF512 (fer-15(b26)II;fem-1(hc17)IV) treated with empty RNAi vector at 15C comparing to at 20C. Two-tailed Student's t-test (n=3, P-value <0.05 was considered significant). WBPaper00057288:Cold_downregulated_protein
  Transcripts that showed significantly increased expression in the germline of iff-1(RNAi) animals comparing to the germine of N2 animals applied with control vector at 15C. Statistical comparisons were made by two-tailed t-test, n=3 (each replicate contains 150 extruded germ lines from N2 wild-type worms), P < 0.01 was considered significant. WBPaper00057288:iff-1(RNAi)_upregulated_transcript
  Transcripts that showed significantly decreased expression in daf-16(mu86);fer-15(b26ts) comparing to in fer-15(b26ts) at 6-day or 7-day post L4 adult hermaphrodite stage. DESeq2 (v1.18.1), ajusted p-value < 0.05 WBPaper00056218:daf-16(mu86)_downregulated_Day7
  Transcripts that showed significantly changed expression in fog-2(q71) comparing to in N2, at 6-day post-L4 adult hermaphrodite comparing and 1-day post L4 adult hermaphrodite animals. Sleuth WBPaper00051558:fog-2(q71)_regulated
Temperature shift: 15C vs 20 C. Transcripts that showed significantly increased expression in N2 animals treated with empty RNAi vector at 15C comparing to N2 animals treated with empty RNAi vector at 20C. Statistical comparisons were made by two-tailed t-test, n=3 (each replicate contains 150 extruded germ lines from N2 wild-type worms), P < 0.01 was considered significant. WBPaper00057288:Cold_upregulated_transcript
  Proteins that showed significantly decreased expression in day 5 adults comparing to young adults. N.A. WBPaper00044746:aging_downregulated_Day5
  Proteins that showed significantly increased expression in day 5 adults comparing to young adults. N.A. WBPaper00044746:aging_upregulated_Day5
  Top 10 transcripts that showed significantly decreased expression in anc-1(RNAi) animals, comparing to N2 animals injected with empty vector. DESeq2 1.22.2, p < 0.0001 WBPaper00060317:anc-1(RNAi)_downregulated
  Neuronal proteins that showed significantly increased expression in day 6 adult comparing to in day 1 adult through in vivo TurboIDlabeling proteomic profiling. DESeq2, log2FC > 1, FDR < 0.05 WBPaper00064789:Day6_vs_Day1_upregulated_neuron
Bacteria diet: Bacillus subtilis Transcripts that showed significantly decreased expression after the formation of B. subtilis biofilm CuffDiff q-value < 0.05. WBPaper00052881:B.subtilis-biofilm_downregulated
  Neuronal proteins that showed significantly decreased expression in day 6 adult comparing to in day 1 adult through in vivo TurboIDlabeling proteomic profiling. DESeq2, log2FC > 1, FDR < 0.05 WBPaper00064789:Day6_vs_Day1_downregulated_neuron
  Top 10 transcripts that showed significantly increased expression in anc-1(RNAi) animals, comparing to N2 animals injected with empty vector. DESeq2 1.22.2, p < 0.0001 WBPaper00060317:anc-1(RNAi)_upregulated
Bacteria diet: Bacillus subtilis Transcripts that showed significantly increased expression after the formation of B. subtilis biofilm CuffDiff q-value < 0.05. WBPaper00052881:B.subtilis-biofilm_upregulated

1 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr12922 Psod-3::GFP appeared to result from a differential expression pattern during aging: in young worms GFP expression is primarily in the head with some expression in the tail, while in older worms the brightest fluorescence occurs around the vulva.  

1 Followed By

Remark Definition Other Name Public Name Primary Identifier
  At 20 Centigrade: 7-8 days after L4-adult molt. 10-11 days after first cleavage. 7-days post-L4 adult hermaphrodite Ce WBls:0000671

1 Preceded By

Remark Definition Other Name Public Name Primary Identifier
  At 20 Centigrade: 5-6 days after L4-adult molt. 8-9 days after first cleavage. 5-days post-L4 adult hermaphrodite Ce WBls:0000068

0 Sub Stages