WormMine: LifeStage WBls:0000068

• Definition: At 20 Centigrade: 5-6 days after L4-adult molt. 8-9 days after first cleavage.
• Primary Identifier: WBls:0000068
• Public Name: 5-days post-L4 adult hermaphrodite Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057
	A C. elegans life stage that occurs during the adult life stage		C. elegans life stage occurring during adulthood	WBls:0000816

268 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 84_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:84_0
	Single-cell RNA-Seq cell group 71_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:71_0
	Transcripts that showed significantly decreased expression at 5-days-post L4 adult N2 hermaphrodites comparing to 1-day-post L4 adult N2 hermaphrodites.	DESeq2, fold change > 2, FDR < 0.05	WBPaper00065835:Day5_vs_Day1_downregulated
	Single-cell RNA-Seq cell group 0_0 expressed in seam.	scVI 0.6.0	WBPaper00065841:0_0
	Single-cell RNA-Seq cell group 21_1 with unidentified tissue expression pattern.	scVI 0.6.0	WBPaper00065841:21_1
Bacteria diet: Lb.rhamnosus	Transcripts that showed significantly decreased expression in animals fed with heterofermentative LAB Lb. rhamnosus, comparing to animals fed with OP50 from day 1 to day 5 adult stage.	fold change > 2 and p value < 0.05 by Students t- test.	WBPaper00065392:Lb.rhamnosus_downregulated
	Transcripts that showed significantly decreased expression in the neurons of bcat-1(RNAi) animals at 5-days post L4 adult hermaphrodite stage, comparing to animals injected with empty vector.	DESeq2. FDR < 0.05.	WBPaper00060459:bcat-1(RNAi)_downregulated
Bacteria diet: Clostridium butyricum MIYAIRI 588 (CBM 588)	Transcripts that showed significantly decreased expression after fed with probiotic bacteria Clostridium butyricum MIYAIRI 588 (CBM 588) for five days, comparing to control groups that were fed with OP50.	DESeq 1.30.0, log2 fold change > 2, p-value < 0.05	WBPaper00055944:C.butyricum_vs_OP50_downregulated
	Transcripts that showed significantly increased expression at 5-days-post L4 adult N2 hermaphrodites comparing to 1-day-post L4 adult N2 hermaphrodites.	DESeq2, fold change > 2, FDR < 0.05	WBPaper00065835:Day5_vs_Day1_upregulated
	Single-cell RNA-Seq cell group 3_4 expressed in intestine.	scVI 0.6.0	WBPaper00065841:3_4
	Single-cell RNA-Seq cell group 3_5 expressed in intestine.	scVI 0.6.0	WBPaper00065841:3_5
	Single-cell RNA-Seq cell group 30_0 expressed in valve.	scVI 0.6.0	WBPaper00065841:30_0
	Single-cell RNA-Seq cell group 5_0 expressed in glia.	scVI 0.6.0	WBPaper00065841:5_0
	Single-cell RNA-Seq cell group 5_1 expressed in glia.	scVI 0.6.0	WBPaper00065841:5_1
	Transcripts that showed significantly increased expression in daf-16(mu86);fer-15(b26ts) comparing to in fer-15(b26ts) at 4-day or 5-day post L4 adult hermaphrodite stage.	DESeq2 (v1.18.1), ajusted p-value < 0.05	WBPaper00056218:daf-16(mu86)_upregulated_Day5
	Single-cell RNA-Seq cell group 43_0 expressed in gonad.	scVI 0.6.0	WBPaper00065841:43_0
	Single-cell RNA-Seq cell group 45_0 expressed in glia.	scVI 0.6.0	WBPaper00065841:45_0
	Genes that increased expression in response to 50uM mianserin on adult day 5, which are not a response to aging. This is a list of 733 genes.	The quasi-likelihood F-test from the edgeR package was used to test these counts for statistically significant differential gene expression between water- and mianserin-treated samples, while controlling for expression differences between the 3 biological replicates. We performed multiple testing correction by using the Benjamini-Hochberg procedure to compute a false discovery rate (FDR) value for each gene, and we considered an FDR less than 10% to be significant.	WBPaper00048926:miaserin_upregulated_adult-day5
	Single-cell RNA-Seq cell group 110_0 expressed in coelomocyte.	scVI 0.6.0	WBPaper00065841:110_0
	Single-cell RNA-Seq cell group 110_1 expressed in mesoderm.	scVI 0.6.0	WBPaper00065841:110_1
	Proteins that showed significantly increased expression in day 5 adults comparing to in day 1 adults at 25C.	fold change > 2	WBPaper00064952:Day5_vs_Day1_upregulated_25C
	Proteins that showed significantly increased expression in day 5 adults comparing to in L4 larva at 25C.	fold change > 2	WBPaper00064952:Day5_vs_L4_upregulated_25C
	Proteins that showed significantly increased expression in day 10 adults comparing to in day 5 adults at 20C.	fold change > 2	WBPaper00064952:Day10_vs_Day5_upregulated_20C
	Single-cell RNA-Seq cell group 141_0 expressed in glia.	scVI 0.6.0	WBPaper00065841:141_0
Bacteria diet: Lb.gasseri	Transcripts that showed significantly decreased expression in animals fed with homofermentative LAB Lb. gasseri, comparing to animals fed with OP50 from day 1 to day 5 adult stage.	fold change > 2 and p value < 0.05 by Students t- test.	WBPaper00065392:Lb.gasseri_downregulated
Bacteria diet: Lb.plantarum	Transcripts that showed significantly decreased expression in animals fed with heterofermentative LAB Lb. plantarum, comparing to animals fed with OP50 from day 1 to day 5 adult stage.	fold change > 2 and p value < 0.05 by Students t- test.	WBPaper00065392:Lb.plantarum_downregulated
Bacteria diet: P.pentosaceus	Transcripts that showed significantly decreased expression in animals fed with heterofermentative LAB P. pentosaceus, comparing to animals fed with OP50 from day 1 to day 5 adult stage.	fold change > 2 and p value < 0.05 by Students t- test.	WBPaper00065392:P.pentosaceus_downregulated
	Single-cell RNA-Seq cell group 25_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:25_0
	Transcripts that showed significantly increased expression in the neurons of bcat-1(RNAi) animals at 5-days post L4 adult hermaphrodite stage, comparing to animals injected with empty vector.	DESeq2. FDR < 0.05.	WBPaper00060459:bcat-1(RNAi)_upregulated
	Single-cell RNA-Seq cell group 9_0 expressed in epithelium.	scVI 0.6.0	WBPaper00065841:9_0

2 Expression Patterns

• Primary Identifier: Expr14942
• Pattern: qRT-PCR analysis revealed that rub-1 was increased with age at transcript levels.

• Primary Identifier: Expr11969
• Pattern: mir-228::GFP expression increases during early adulthood, reaches a peak, and then begins to slowly decrease in mid to late adulthood.

2 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	At 20 Centigrade: 6-7 days after L4-adult molt. 9-10 days after first cleavage.		6-days post-L4 adult hermaphrodite Ce	WBls:0000670
	At 20 Centigrade: 4-7 days after L4-adult molt. 7-10 days after first cleavage.		4-7 days post-L4 adult hermaphrodite Ce	WBls:0000069

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	At 20 Centigrade: 96-120 hours after L4-adult molt. 7-8 days after first cleavage.		4-days post-L4 adult hermaphrodite Ce	WBls:0000067

0 Sub Stages