WormMine: Gene WBGene00009342

• WormBase Gene ID: WBGene00009342
• Gene Name: fasn-1
• Sequence Name: F32H2.5
• Brief Description: fasn-1 encodes a fatty acid synthase, orthologous to human FASN (OMIM:600212); CEP-1 is required for fully normal fasn-1 expression in vivo; CEP-1 drives transcription of luciferase reporters whose promoters contains either of two putative CEP-1 binding sites (FAS-T1 and FAS-T2) found in the fasn-1 gene, and this activity is partially lost bymutation of conserved CEP-1 residues (R298 or H310); in humans, the CEP-1 ortholog isoforms TAp73alpha and deltaNp63alpha bind the human FASN gene, suggesting that FASN genes might be a conserved direct target of p53-like proteins in metazoa; fasn-1 transcription is moderately activated (2 to 4-fold) in L1 or L2 larvae starved for 12 hours, but is not so activated in later larvae or adults.
• Organism: Caenorhabditis elegans
• Automated Description: Predicted to enable fatty acid synthase activity. Predicted to be involved in fatty acid biosynthetic process. Expressed in excretory duct; hermaphrodite gonad; hypodermis; intestine; and neurons. Used to study muscular disease. Human ortholog(s) of this gene implicated in colorectal cancer. Is an ortholog of human FASN (fatty acid synthase).
• Biotype: SO:0001217
• Genetic Position: I :3.34704 ±0.00214
• Length (nt): 11175

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Synonyms

• Value: WBGene00009342

Genomics

1 Transcripts

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
Transcript:F32H2.5.1	F32H2.5.1	8451	I: 8975366-8986540

Other

1 CDSs

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F32H2.5	F32H2.5	7842	I: 8975957-8976049

40 RNAi Result

• WBRNAi00082110
• WBRNAi00085606
• WBRNAi00090850
• WBRNAi00082107
• WBRNAi00095245
• WBRNAi00102780
• WBRNAi00110682
• WBRNAi00077423
• WBRNAi00077425
• WBRNAi00077424
• WBRNAi00000042
• WBRNAi00025364
• WBRNAi00025365
• WBRNAi00046163
• WBRNAi00116623
• WBRNAi00117454
• WBRNAi00092779
• WBRNAi00092794
• WBRNAi00086008
• WBRNAi00092775
• WBRNAi00064422
• WBRNAi00095242
• WBRNAi00095241
• WBRNAi00081353
• WBRNAi00102656
• WBRNAi00095860
• WBRNAi00095937
• WBRNAi00096012
• WBRNAi00096089
• WBRNAi00096166

115 Allele

• gk962858
• gk962706
• gk963902
• gk963849
• gk964316
• h12037
• gk964252
• WBVar01432411
• WBVar01432410
• WBVar01712986
• fr8
• g43
• g14
• gk117855
• gk117856
• gk117857
• gk117858
• gk117852
• WBVar00538216
• gk117853
• gk117854
• gk117863
• gk117864
• gk117865
• gk117866
• gk117859
• gk117860
• fr7
• gk117861
• gk117862

1 Chromosome

• WormBase ID: I
• Organism: Caenorhabditis elegans
• Length (nt): 15072434

1 Chromosome Location

• Feature . Primary Identifier: WBGene00009342
• Start: 8975366
• End: 8986540
• Strand: -1

4 Data Sets

• WormBaseAcedbConverter
• GO Annotation data set
• C. elegans genomic annotations (GFF3 Gene)
• Panther orthologue and paralogue predictions

1 Downstream Intergenic Region

• WormBase ID: intergenic_region_chrI_8975117..8975365
• Length (nt): 249
• Chromosome Location: I: 8975117-8975365
• Organism: Caenorhabditis elegans

227 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	oocyte proteins identified by two or more unique peptides during proteomics study.	In the pooled data set, 1453 C. elegans proteins were identified with a probability >= 0.9 according to ProteinProphet, of which 1165 proteins were identified by more than one unique peptide.	WBPaper00038289:oocyte_protein
	Transcripts of coding genes that showed significantly decreased expression in muscle.	DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed.	WBPaper00062325:muscle_depleted_coding-RNA
	Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons.	DESeq. False discovry rate (FDR) < 0.1.	WBPaper00048988:neuron_expressed
adult vs dauer larva	Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C.	N.A.	WBPaper00050488:adult_vs_dauer_regulated_N2_20C
	TGF- Dauer pathway adult transcriptional targets. Results obtained by comparing the microarray results of the dauer-constitutive mutants daf-7(e1372), daf-7(m62), and daf-1(m40) with dauer-defective mutants daf-3(mgDf90), daf-5(e1386), and daf-7(e1372);daf-3(mgDf90) double mutants at the permissive temperature, 20C, on the first day of adulthood.	SAM	WBPaper00031040:TGF-beta_adult_upregulated
	Transcripts that showed significantly increased expression glp-1(e2141); TU3401 animals comparing to in TU3401 animals.	Fold change > 2, FDR < 0.01.	WBPaper00065993:glp-1(e2141)_upregulated
	Transcripts that showed significantly increased expression after animals were treated with 50uM Rifampicin from day 1 to day 3 adult hermaphradite.	DESeq2(v1.14.1), fold change > 2, p-value < 0.05	WBPaper00055354:Rifampicin_upregulated
	Proteins interacting with NHR-49-GFP according to co-IP and LC-MS.	N.A.	WBPaper00064071:NHR-49_interacting
	Transcripts expressed in the epithelial tissues surrounding the pharynx that includes the arcade and intestinal valve (AIV) cells, according to PAT-Seq analysis using Pbath-15-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:arcade_intestinal-valve_expressed
	Transcripts expressed in body muscle, according to PAT-Seq analysis using Pmyo-3-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:body-muscle_expressed
	Transcripts expressed in GABAergic neuron, according to PAT-Seq analysis using Punc-47-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:GABAergic-neuron_expressed
	Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:hypodermis_expressed
	Transcripts that showed significantly decreased expression in atfs-1(cmh15) (null allele) animals comparing to in N2 animals at L4 larva stage.	edgeR, fold change > 2, FDR < 0.05	WBPaper00060909:atfs-1(cmh15)_downregulated
	Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:intestine_expressed
	Transcripts expressed in seam cells, according to PAT-Seq analysis using Pgrd-10-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:seam_expressed
	Single-cell RNA-Seq cell group 0_0 expressed in seam.	scVI 0.6.0	WBPaper00065841:0_0
	Transcripts detected in germline isolated from day-1 adult hermaphrodite animals.	All three experiments have CPM >= 1.	WBPaper00067147:germline_expressed
Bacteria infection: Bacillus thuringiensis	mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 10 mix) vs BT407 12h), according to RNAseq.	Cuffdiff, ajusted p-value < 0.01.	WBPaper00046497:B.thuringiensis_0.1mix_downregulated_12h
	Transcripts that showed significantly increased expression in sin-3(tm1276) comparing to in N2.	DESeq2, fold change > 2, p-value < 0.01.	WBPaper00061203:sin-3(tm1276)_upregulated
Bacteria infection: Bacillus thuringiensis	mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 12h), according to RNAseq.	Cuffdiff, ajusted p-value < 0.01.	WBPaper00046497:B.thuringiensis_0.5mix_downregulated_12h
	Single-cell RNA-Seq cell group 21_1 with unidentified tissue expression pattern.	scVI 0.6.0	WBPaper00065841:21_1
Bacteria diet: Comamonas aquatica DA1887 vs. E. coli OP50	Transcripts that showed significantly decreased expression in N2 L3 larva animals fed with Comamonas aquatica strain DA1887, comparing to in N2 L3 larva animals fed with E. coli OP50.	edgeR, fold change > 2, p-value < 0.05.	WBPaper00067248:C.aquatica_downregulated_L3
	Transcripts that showed significantly increased expression in mrg-1(qa6200) comparing to in control animals in primordial germ cells (PGCs) at L1 larva stage.	DESeq2(v1.32.0), FDR < 0.05.	WBPaper00064315:mrg-1(qa6200)_upregulated_PGCs
	Transcripts that showed significantly increased expression in hrde-1(tm1200) animals, comparing to in N2, after growing at 25C for five generations (late generation).	CuffDiff2	WBPaper00051265:F4_hrde-1(tm1200)_upregulated
	Transcripts that showed significantly increased expression in aak-1(tm1944);aak-2(ok524) animals comparing to in N2.	DEseq 1.18.0, adjusted p-value < 0.05.	WBPaper00056471:aak-1(tm1944);aak-2(ok524)_upregulated
	Transcripts that showed significantly decreased expression in N2 animals exposed to 0.1mM Paraquat from hatching to reaching adult stage.	DESeq2 version 1.22.2, p < 0.05	WBPaper00064716:paraquat_downregulated
	Transcripts that showed signifcantly increased expression in ubr-5(miy31) injected with empty vector comparing to in N2 animals injected with empty vector at L1 larva stage and collected at L4 larva stage.	FDR < 0.05, fold change > 2	WBPaper00067368:ubr-5(miy31)_upregulated_N2
Bacteria infection: Staphylococcus aureus	Transcripts that showed significantly decreased expression in animals experimentally colonised by a wild microbiota community and infected by the widespread animal pathogen, Staphylococcus aureus, comparing to animals not colonized by microbiota and not infected by pathogen.	DeSeq2 (v. 1.42.0), Wald analyses testing against a null hypothesis of < |1.5|-fold change in gene expression between treatments (BenjaminiHochberg adjusted false detection rate of p <= 0.05.	WBPaper00067479:Microbiota-Pathogen_vs_control_downregulated
Bacteria infection: Staphylococcus aureus	Transcripts that showed significantly decreased expression in animals experimentally colonised by a wild microbiota community and infected by the widespread animal pathogen, Staphylococcus aureus, comparing to animals colonized by microbiota but not infected by pathogen.	DeSeq2 (v. 1.42.0), Wald analyses testing against a null hypothesis of < |1.5|-fold change in gene expression between treatments (BenjaminiHochberg adjusted false detection rate of p <= 0.05.	WBPaper00067479:Microbiota-Pathogen_vs_Microbiota_downregulated
	Transcripts that showed significantly decreased expression in N2 day 5 adults comparing to in N2 day 1 adult animals according to Illumina RNASeq.	DESeq fold change > 2, FDR < 0.05.	WBPaper00067499:Day5_vs_Day1_downregulated_Illumina

8 Expression Patterns

• Primary Identifier: Expr1034087
• Pattern: Tiling arrays expression graphs

• Primary Identifier: Expr1150053
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015

• Primary Identifier: Expr11679
• Pattern: Expression of fasn-1 was visible in the intestine, spermatheca, many head and tail neurons, in the main epidermal syncytium hyp7 and the seam cells.

• Primary Identifier: Expr15197
• Pattern: fasn-1 was labeled with GFP at the amino terminus (tn1782) or carboxyl-terminus (av138). Both alleles exhibited similar GFP expression patterns. GFP is seen in the hypodermis, gonad sheath (esp. proximal) and excretory duct; however, intestine and CAN neuron expression are not evident. Neither GFP::POD-2 nor GFP-labelled FASN-1 is expressed in the DTC. No germline expression of any of these GFP-tagged genes was detected, including by anti-GFP staining of dissected gonads. This was most clearly observed by examination of the distal gonad; however, abundant GFP::POD-2 or FASN-1::GFP expression in the proximal sheath cells made it more difficult to evaluate expression in the proximal germline. The earliest detectable GFP-tagged FASN-1 in embryos appears in developing hypodermis.

• Primary Identifier: Expr9290
• Remark: Reporter gene fusion type not specified.
• Pattern: fasn-1 is expressed exclusively in the main body syncytium (hyp-7) throughout all larval stages.

• Primary Identifier: Expr2011433
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

• Primary Identifier: Expr1022462
• Pattern: Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012

• Primary Identifier: Expr2029671
• Pattern: Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).

21 GO Annotation

7 Homologues

1 Locations

• Feature . Primary Identifier: WBGene00009342
• Start: 8975366
• End: 8986540
• Strand: -1

21 Ontology Annotations

4 Regulates Expr Cluster

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts that showed significantly decreased expression in starved fasn-1(g14) animals comparing to in starved N2 animals.	edgeR 3.28, fold change > 2, FDR < 0.05.	WBPaper00064721:fasn-1(g14)_downregulated_starved
	Transcripts that showed significantly increased expression in animals overexpressing the c-terminal fragment of FASN-1 (FASN-CTF).	fold change > 2, FDR < 0.05	WBPaper00066349:fasn-1(overexpress)_downregulated
	Transcripts that showed significantly increased expression in starved fasn-1(g14) animals comparing to in starved N2 animals.	edgeR 3.28, fold change > 2, FDR < 0.05.	WBPaper00064721:fasn-1(g14)_upregulated_starved
	Transcripts that showed significantly increased expression in animals overexpressing the c-terminal fragment of FASN-1 (FASN-CTF).	fold change > 2, FDR < 0.05	WBPaper00066349:fasn-1(overexpress)_upregulated

1 Sequence

• Length: 11175

1 Sequence Ontology Term

• Name: gene

6 Strains

• WBStrain00021979
• WBStrain00049318
• WBStrain00054637
• WBStrain00062782
• WBStrain00007838
• WBStrain00007819

1 Upstream Intergenic Region

• WormBase ID: intergenic_region_chrI_8986541..8987094
• Length (nt): 554
• Chromosome Location: I: 8986541-8987094
• Organism: Caenorhabditis elegans