WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Gene :

WormBase Gene ID  ? WBGene00003912 Gene Name  pal-1
Sequence Name  ? C38D4.6 Brief Description  pal-1 encodes a homeodomain protein that is the C. elegans Caudal ortholog; PAL-1 is required maternally and embryonically for specification of the C and D posterior blastomere fates and thus, for proper bodywall muscle and hypodermal development during embryogenesis; PAL-1 is also required postembryonically for fate specification of the posterior V6 neuroectoblast during male tail development; in regulating embryonic bodywall muscle specification, PAL-1 binds to an enhancer element in the promoter of hlh-1, the sole C. elegans MRF family member, to positively regulate hlh-1 transcription; PAL-1 is expressed in posterior blastomeres beginning at the four-cell stage of embryogenesis and its expression continues during embryogenesis in posterior-derived tissues and cells undergoing movement or forming midline structures; pal-1 is subject to both transcriptional and translational regulation.
Organism  Caenorhabditis elegans Automated Description  Enables DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in several processes, including cell fate commitment; embryonic pattern specification; and positive regulation of DNA-templated transcription. Located in condensed nuclear chromosome. Expressed in several structures, including ABprpppppaa; Caaaaa; enteric muscle; epithelial cell; and tail spike. Human ortholog(s) of this gene implicated in Barrett's esophagus. Is an ortholog of human CDX1 (caudal type homeobox 1) and CDX2 (caudal type homeobox 2).
Biotype  SO:0001217 Genetic Position  III :-2.46048 ±0.0149
Length (nt)  ? 4889
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Synonyms

Value
WBGene00003912

Genomics

5 Transcripts

WormMine ID Sequence Name Length (nt) Chromosome Location
Transcript:C38D4.6c.1 C38D4.6c.1 1192   III: 4804688-4808094
Transcript:C38D4.6b.2 C38D4.6b.2 1321   III: 4804706-4808717
Transcript:C38D4.6b.1 C38D4.6b.1 1424   III: 4804707-4809553
Transcript:C38D4.6a.1 C38D4.6a.1 1317   III: 4804708-4808709
Transcript:C38D4.6a.2 C38D4.6a.2 1451   III: 4804709-4809576
 

Other

3 CDSs

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:C38D4.6a C38D4.6a 813   III: 4805211-4805253
CDS:C38D4.6c C38D4.6c 669   III: 4805211-4805253
CDS:C38D4.6b C38D4.6b 807   III: 4805211-4805253

77 RNAi Result

WormBase ID
WBRNAi00095522
WBRNAi00114286
WBRNAi00114051
WBRNAi00114130
WBRNAi00114209
WBRNAi00082742
WBRNAi00106570
WBRNAi00066939
WBRNAi00066966
WBRNAi00067021
WBRNAi00067156
WBRNAi00067181
WBRNAi00067251
WBRNAi00067313
WBRNAi00067337
WBRNAi00067405
WBRNAi00067435
WBRNAi00067440
WBRNAi00067658
WBRNAi00067738
WBRNAi00067812
WBRNAi00068118
WBRNAi00068137
WBRNAi00042160
WBRNAi00042161
WBRNAi00103906
WBRNAi00103905
WBRNAi00103908
WBRNAi00114247
WBRNAi00103907

71 Allele

Public Name
WBVar01607021
WBVar01541723
WBVar01656569
WBVar01656568
ns115
ns114
WBVar01995073
WBVar01644290
WBVar01644291
WBVar01644292
WBVar01644293
ttTi5621
WBVar01785633
WBVar01566363
e2091
WBVar01903723
WBVar02074211
WBVar01628474
WBVar01852293
gk173458
ok690
WBVar01445471
WBVar01445470
WBVar01396760
WBVar01396759
gk818627
gk173464
ct224
gk620526
gk173463

1 Chromosome

WormBase ID Organism Length (nt)
III Caenorhabditis elegans 13783801  

1 Chromosome Location


Feature . Primary Identifier
Start End Strand
WBGene00003912 4804688 4809576 -1

4 Data Sets

Name URL
WormBaseAcedbConverter  
GO Annotation data set  
C. elegans genomic annotations (GFF3 Gene)  
Panther orthologue and paralogue predictions  

0 Downstream Intergenic Region

180 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Genes with expression altered >= 3-fold in dpy-10(e128) mutants. Data across the wild type series was analyzed using the Significance analysis of Microarrays (SAM) algorithm (to calculate the False Discovery Rate (FDR)). WBPaper00035873:dpy-10_regulated
  Transcripts of coding genes that showed significantly decreased expression in muscle. DESeq2 (version 1.24.0). Transcripts with a false-discovery rate adjusted p-value less than 0.05 were considered significantly differentially expressed. WBPaper00062325:muscle_depleted_coding-RNA
  Transcripts expressed in neuronal cells, by analyzingfluorescence-activated cell sorted (FACS) neurons. DESeq. False discovry rate (FDR) < 0.1. WBPaper00048988:neuron_expressed
adult vs dauer larva Transcripts that showed differential expression in adult vs dauer lava in N2 animals at 20C. N.A. WBPaper00050488:adult_vs_dauer_regulated_N2_20C
Bacteria infection: Enterococcus faecalis OG1RF. Exposure for 16 hours. Transcripts that showed significantly decreased expression in N2 after animals were exposed to E. faecalis OG1RF for 16 hours comparing to exposure to E. Coli OP50. Cuffcompare and Cuffdiff WBPaper00056090:E.faecalis_downregulated_N2
  Transcripts expressed in body muscle, according to PAT-Seq analysis using Pmyo-3-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:body-muscle_expressed
  Transcripts expressed in hypodermis, according to PAT-Seq analysis using Pdpy-7-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:hypodermis_expressed
  Transcripts expressed in intestine, according to PAT-Seq analysis using Pges-1-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:intestine_expressed
  Maternal class (M): genes that are called present in at least one of the three PC6 replicates. A modified Welch F statistic was used for ANOVA. For each gene, regressed error estimates were substituted for observed error estimates. The substitution is justified by the lack of consistency among the most and least variable genes at each time point. Regressed error estimates were abundance-dependent pooled error estimates that represented a median error estimate from a window of genes of similar abundance to the gene of interest. A randomization test was used to compute the probability Pg of the observed F statistic for gene g under the null hypothesis that developmental time had no effect on expression. P-values were not corrected for multiple testing. [cgc5767]:expression_class_M
  Transcripts expressed in pharynx, according to PAT-Seq analysis using Pmyo-2-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:pharynx_expressed
  Genes with expression level regulated by genotype (N2 vs CB4856) and age at L3 larva and Late reproduction stage (96 hours at 24 centigrade). For model 2, authors used 100 permutations to estimate the FDR threshold. Per permutation, genotypes and ages were independently randomly distributed, keeping the among-gene structure intact. Then for each spot (23,232) on the array, model 2 was tested. The obtained P-values were used to estimate a threshold for each of the explanatory factors. Authors also used a genome-wide threshold of -log10 P-value = 2, which resembles an FDR of 0.072 and 0.060 for marker and the interaction age-marker for the developing worms and FDR of 0.050 and 0.065 for marker and age-marker for the aging worms. For the physiological age effect, authors used a log10 P-value = 8 in developing worms (0.012 FDR) and -log10 P-value = 6 (0.032 FDR). WBPaper00040858:eQTL_age_regulated_developing
  Transcripts expressed in seam cells, according to PAT-Seq analysis using Pgrd-10-GFP-3XFLAG mRNA tagging. Cufflinks FPKM value >=1. WBPaper00050990:seam_expressed
  Transcripts that showed significantly increased expression in day 1 adult hermaphrodite comparing to in L4 larva fem-3(q20) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-1-adult_vs_L4_upregulated_fem-3(q20)
  Transcripts that showed significantly increased expression in day 3 adult hermaphrodite comparing to in L4 larva fem-3(q20) animals. Fold change > 2, FDR < 0.05 WBPaper00064088:Day-3-adult_vs_L4_upregulated_fem-3(q20)
Bacteria diet: Sphingomonas aquatilis Yellow. Fed for 30 generations. Transcripts that showed significantly decreased expression after fed by bacteria Sphingomonas aquatilis (Yellow) for 30 generations comparing to animals fed by E. coli OP50. DESeq2 fold change > 2, p-value < 0.01. WBPaper00061007:S.aquatilis_downregulated
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 10 mix) vs BT407 6h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.1mix_downregulated_6h
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 10 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.1mix_downregulated_12h
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 6h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_6h
Bacteria infection: Bacillus thuringiensis mRNAs that showed significantly decreased expression after pathogenic bacteria Bacillus thuringiensis infections comparing to non pathogenic BT (BT247(1 to 2 mix) vs BT407 12h), according to RNAseq. Cuffdiff, ajusted p-value < 0.01. WBPaper00046497:B.thuringiensis_0.5mix_downregulated_12h
  Transcripts that showed significantly decreased expression in hsp-6(mg585) comparing to in N2 at L4 larva stage. EdgeR, fold change > 2, FDR < 0.001. WBPaper00056290:hsp-6(mg585)_downregulated
Heat Shock: 35C 4 hours at L4 larva stage. Transcripts that showed significantly decreased expression after L4 larva N2 animals were heat stressed at 35C for 4 hours DESeq2 WBPaper00057154:HeatShock_downregulated_mRNA
  Genes down regulated by mir-243(n4759). RNAs that changed at least 2-fold with a probability of p > 0.05 in three biological replicates were considered differentially regulated between wild-type and mir-243. WBPaper00036130:mir-243_down_regulated
25C vs. 20C Transcripts that showed significantly increased expression in 1-day post L4 adult hermaphrodite N2 grown at 25C, comparing to in N2 animals grown at 20C. CuffDiff, fold change > 2. WBPaper00065096:25C_vs_20C_upregulated
  Transcripts that showed significantly increased expression in 10-days post L4 adult hermaphrodite N2 grown at 20C, comparing to in 1-day post L4 adult hermaphrodite N2 animals grown at 20C. CuffDiff, fold change > 2. WBPaper00065096:Day10_vs_Day1_upregulated
  Genes with increased RNA expression after 24 hours rotenone treatment EdgeR provides statistical routines for determining differential expression in digital gene expression data using a model based on the negative binomial distribution. The resulting p-values were adjusted using the Benjamini and Hochbergs approach for controlling the false discovery rate (FDR). Transcripts with an adjusted p-value smaller 0.05 were assigned as differentially expressed. WBPaper00044426:rotenone_24h_upregulated
  Transcripts that showed significantly decreased expression in tetraploid N2 comparing to diploid N2 animals at L4 larva stage. DESeq2 R package (1.20.0), fold change > 2, and FDR < 0.05. WBPaper00066110:tetraploid_vs_diploid_downregulated
  Transcripts that showed significantly decreased expression in hpl-2(tm1489) comparing to in N2 animals. DESeq2, adjusted p-value < 0.05, log2 fold change > 2 or < -2. WBPaper00054493:hpl-2(tm1489)_downregulated
  Transcripts that showed significantly increased expression in ilc-17.1(syb5296) comparing to in N2 animals at L4 larva stage. DESeq2, fold change > 2, FDR < 0.05. WBPaper00066594:ilc-17.1(syb5296)_upregulated
  Transcripts that showed significantly decreased expression after animals were treated with 50uM Rifampicin from day 1 to day 3 adult hermaphradite. DESeq2(v1.14.1), fold change > 2, p-value < 0.05 WBPaper00055354:Rifampicin_downregulated
Bacteria: B.thuringiensis Transcripts in elt-2(RNAi) animals that were significantly differentially expressed at least for one time point and one pathogenic strain Bt247 and Bt679 compared to the non pathogenic strain Bt407. Cuffdiff WBPaper00060358:B.thuringiensis_pathogen_regulated_elt-2(RNAi)

21 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr1031846 Tiling arrays expression graphs  
    Expr7324 Click the movie links below for interactive 4D movies of a fluorescence reporter. http://glowormnotes.blogspot.com/2009/11/pal-1yfp-transcriptional-fusion.html  
early embryo(author) = blastula embryo(curator).   Expr550 PAL-1 protein was detected in all P1 descendants from the 4-cell through the 24-cell stage. Staining was variable at the 24-cell stage. At the 28-cell stage, PAL-1 was detected in all P2 descendants.  
    Expr3226 A pal-1 promoter::YFP fusion is expressed exclusively in the C and D lineage of early wild-type embryos.  
Three methods, lacZ, gfp, antibody staining results all mixed together. Lots of unextracted cell objects buried in pattern text.   Expr841 PAL-1 produced from zygotic transcripts is seen initially in C and D lineage cells that also expressed maternally derived PAL-1. As gastrulation begins, expression is seen in only Ca and Cp and then in their daughters, of which 2 are hypodermoblasts (Caa and Cpa) and 2 are myoblasts (Cap and Cpp). The GFP reporter is first detected at the late 2C-cell stage and then more strongly in the 4 daughters. At about 100 cells, expression is also detected in the 2 D-lineage myoblasts. Thereafter, PAL-1 continues to be detected in all C and D descendants until the end of gastrulation at about 350 cells. At about 180 cells (midgastrulation), the C hypodermal precursors, which express more strongly than the muscle precursors, form a characteristic double row on each side of the dorsal midline in the posterior. Thereafter, PAL-1 decreases in these cells and is no longer detectable with antibody after 350 500 cells. At about 250 cells, expression is detected in two AB cells that border the posterior left edge of the mesectodermal cell layer that is closing the ventral gastrulation cleft (ABplpappp and ABplppppp) and slightly later in the right homolog of one of them (ABprppppp). The daughters and granddaughters of these cells, generated after the cleft closes, continue to express strongly along the ventral midline until about the time of hatching. Beginning at about 360 cells, as morphogenesis begins, weak transient expression is detected in the posterior ectodermal P cells and occasionally in posterior V cells as both groups move ventrally. During this period the V cells become the lateral seam cells, and the P cells undergo their terminal embryonic divisions as they complete hypodermal enclosure of the embryo. Meanwhile, in the interior, pal-1 expression, detectable both with antibody and with reporter constructs, appears at about 350 cells in 2 Ea descendents near the middle of the gut primordium (the int5 pair) and in 2 anteriorly located MS descendants which migrate to the posterior and become the mesoblast M and the right intestinal muscle (mu intR). During early morphogenesis as the embryo develops through the comma stage and begins to elongate, all the pal-1-expressing cells (approximately 50) are located in the posterior ventral region, except for the 2 midgut cells which lie more dorsally. The descendants of ABpl/rppppp, as well as mu intR, move into the elongating tail and participate in formation of the rectal and associated intestinal muscles, as well as the ventral tail hypodermis. Expression diminishes during elongation and by hatching is detectable only in the 2 gut cells, M, mu intR, and 10 cells descended from ABpl/rppppp.  
    Expr10516 Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
    Expr10517 Inferred Expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
See Expr746 for in situ data. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).   Expr698 High levels in P2 and EMS (posterior nuclei) at the 4-cell stage. From 4-cell to the 24-cell stage, PAL-1 is detected in all descendants of both P2 and EMS. The PAL-1 antibody stained the nuclei throughout the cell cycle and was localized to the condensed chromosomes during mitosis. At the 24-cell stage, the level of staining intensity increased in the two C descendants, Ca and Cp. In 28-cell embryos strong staining was observed in the four C descendants and lower levels of staining was also observed in D and P4. At the 28-cell stage expression was stronger in P2 than in EMS descendants.  
    Expr10401 Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).   Expr746 pal-1 RNA evenly distributed in 1- and 2-cell embryos. In 4-cell embryos, pal-1 RNA was either uniformly distributed (approximately 50%) or preferentially localized to the posterior blastomere. Nuclear localized transcripts were not detected before the 24-cell stage. In 6 cell and older embryo, pal-1 RNA was more abundant in cells that express PAL-1 protein.  
    Expr11958 PAL-1 is expressed in wild-type gonads.  
  We employed a transgene-based assay that tests the post-transcriptional control activity of 3' UTRs in vivo. In this assay, the GFP::H2B reporter fusion is placed upstream of the selected 3' UTR such that the entire fusion-GFP::H2B-3' UTR-is produced as a single transcript. Expression in the germline is achieved by using pie-1 promoter, which has been shown earlier to drive transgene expression in the germline. Expr9714 The transgene containing pal-1 3' UTR expressed the GFP::H2B reporter strongly in oocytes, but not in mitotic or early pachytene regions.  
    Expr16038 We detected embryonic expression of our rescuing pal-1::gfp construct, containing the full genomic sequence of pal-1, in two pairs of epidermal daughter cells, V3 and P7/8, and V4 and V6, from shortly after their birth. In contrast, we were unable to detect any expression of pal-1 in L1 larvae, supporting the hypothesis that pal-1 functions in embryos to coordinate L1 ventral epithelial morphogenesis.  
    Expr1200139 Data from the TransgeneOme project  
    Expr1146158 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/hashimshony2015  
    Expr2032921 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  
    Expr15634    
    Expr1027672 Developmental gene expression time-course. Raw data can be downloaded from ftp://caltech.wormbase.org/pub/wormbase/datasets-published/levin2012  
    Expr10402 Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
    Expr10403 Inferred expression. EPIC dataset. http://epic.gs.washington.edu/ Large-scale cellular resolution compendium of gene expression dynamics throughout development. This reporter was inferred to be expressing in this cell or one of its embryonic progenitor cells as described below. To generate a compact description of which cells express a particular reporter irrespective of time, the authors defined a metric "peak expression" for each of the 671 terminal ("leaf") cells born during embryogenesis. For each of these cells, the peak expression is the maximal reporter intensity observed in that cell or any of its ancestors; this has the effect of transposing earlier expression forward in time to the terminal set of cells. This metric allows straightforward comparisons of genes' cellular and lineal expression overlap, even when the expression occurs with different timing and despite differences in the precise time point that curation ended in different movies, at the cost of ignoring the temporal dynamics of expression, a topic that requires separate treatment. For simplicity, the authors use the term "expressing cells" to mean the number of leaf cells (of 671) with peak expression greater than background (2000 intensity units) and at least 10% of the maximum expression in that embryo. Quantitative expression data for all cells are located here: ftp://caltech.wormbase.org/pub/wormbase/datasets-published/murray2012/  
    Expr2014688 Single cell embryonic expression. Only cell types with an expression fraction of greater 0.2 of the maximum expressed fraction are labeled (Full data can be downloaded from http://caltech.wormbase.org/pub/wormbase/datasets-published/packer2019/). The colors represent the broad cell class to which the cell type has been assigned. The size of the point is proportional to the log2 of the numbers of cells in the dataset of that cell type. Interactive visualizations are available as a web app (https://cello.shinyapps.io/celegans/) and can also be installed as an R package (https://github.com/qinzhu/VisCello.celegans).  

29 GO Annotation

Annotation Extension Qualifier
  enables
  enables
  enables
  enables
has_input(WB:WBGene00000417) enables
  enables
  enables
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  located_in
  located_in
  involved_in
  located_in
  involved_in
has_input(WB:WBGene00000417) involved_in
  involved_in
  involved_in
  located_in
  located_in
  located_in
  located_in
  located_in
  located_in

12 Homologues

Type
orthologue
orthologue
orthologue
orthologue
least diverged orthologue
least diverged orthologue
least diverged orthologue
orthologue
orthologue
orthologue
least diverged orthologue
least diverged orthologue

1 Locations


Feature . Primary Identifier
Start End Strand
WBGene00003912 4804688 4809576 -1

29 Ontology Annotations

Annotation Extension Qualifier
  enables
  enables
  enables
  enables
has_input(WB:WBGene00000417) enables
  enables
  enables
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  involved_in
  located_in
  located_in
  involved_in
  located_in
  involved_in
has_input(WB:WBGene00000417) involved_in
  involved_in
  involved_in
  located_in
  located_in
  located_in
  located_in
  located_in
  located_in

2 Regulates Expr Cluster

Regulated By Treatment Description Algorithm Primary Identifier
  Potential PAL-1 target genes. Clustering, correlation to known target genes and ANOVA were used to score the PAL-1 target potential of each gene. WBPaper00025032:PAL-1_target_genes
  Transcription factors that showed significantly incresed expression 2 hours after heat shock PAL-1[JA1179] at 34C for 30min. Genes encoding possible transcription factors that were up-regulated at least 5-fold at 2 hrs post-induction of the specified transcription factor. WBPaper00028867:PAL-1_induced_TF

1 Sequence

Length
4889

1 Sequence Ontology Term