WormMine: ExpressionPattern Expr4260

• Pattern: Prominent expression of STIM-1::GFP was detected in the spermatheca, gonad sheath cells, the intestine, and neurons in the head. Expression was also detected in uterine epithelial cells. STIM-1::GFP-expressing head neurons are likely amphid and/or inner labial (IL) neurons. Expression of STIM-1::GFP in the intestine was heterogeneous. The anterior and posterior intestine expressed the reporter very strongly while expression was weaker in the midsection.
• Primary Identifier: Expr4260
• Remark: More detailed studies are required to identify the cellular domains in which STIM-1::GFP is expressed. It should be noted here that the absence of detectable STIM-1::GFP expression in tissues other than those shown does not rule out a functional role for STIM-1 in other cell types. The 1.9-kb stim-1 promoter used in these studies may lack regulatory information required for cell-specific expression. In addition, STIM-1::GFP expression levels may be below detection levels in other tissues. More definitive identification of STIM-1 expression sites awaits the development of suitable antibodies for immunolocalization.
• Subcellular Localization: Intestinal STIM-1::GFP appeared to be localized to membrane and submembrane regions. Confocal Z-sections also revealed a prominent punctate localization in the anterior intestine and sheath cells. STIM-1::GFP expression showed a striking localization to a reticular structure in the posterior intestine. This reticular structure resembles the ER of C. elegans intestinal cells.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
neuron of the amphid sensillum	amphid neuron	amphid sensory neuron	WBbt:0005394
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828
toroidal epithelial cell that make up the bulk of the uterus, four cells anterior and four cells posterior to the vulva.	uterine toroidal epithelial cell	ut cell	WBbt:0006784
neuron type, member of a set of 12 neurons of the inner labial sensilla.	inner labial neuron	IL neuron	WBbt:0005117

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00021910	stim-1	Y55B1BM.1	Caenorhabditis elegans

0 Life Stages