WormMine: ExpressionPattern Expr2688

• Pattern: Immunolocalization of UNC-78 revealed that UNC-78 is expressed in a tissue-specific manner. In embryos, faint staining of UNC-78 was first detected in a subset of cells at the 1.5-fold stage (~350 minutes after the first cell division). In the later embryonic stages, UNC-78 was expressed in the body wall muscle and also more strongly in the pharynx. In adults, UNC-78 was expressed in the body wall muscle, pharynx and spermatheca.
• Primary Identifier: Expr2688
• Remark: Staining of unc-78 null mutants with anti-UNC-78 antibody did not yield these patterns, indicating that striated staining is specific for reactivity with UNC-78.
• Subcellular Localization: In 1.5-fold embryos: The UNC-78-positive regions were adjacent to the nascent myofibrils in body wall muscle where the muscle-specific myosin heavy chain myoA was localized, suggesting that UNC-78 is localized in the diffuse cytoplasm of the body wall muscle cells. In later embryos, in the pharynx and spermatheca, subcellular localization of UNC-78 was not clear owing to poor penetration of the antibody into these tissues. In the body wall muscle, UNC-78 was localized in a striated pattern that was co-localized with actin but not with the myosin heavy chain myoA. However, since the striation of UNC-78 was not as sharp as that of actin, it was difficult to determine precise location of UNC-78 within the thin filaments.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006810	unc-78	C04F6.4	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018