WormMine: ExpressionPattern Expr12087

• Pattern: GFP expression is detectable in comma stage embryos with strongest expression in the tail region. The distinct subcellular localization makes it difficult to confirm cell identities, but overall expression is comparable to the plr-1::GFP promoter construct. Postembryonical GFP expression in the head region seems restricted to some neurons and the posterior region of the pharynx. Expression was not readily detectable in hypodermal or body wall muscle cells with this construct postembryonically. Expression of the PLR-1::GFP fusion protein, however, is detectable in the AVG neuron and also in the excretory cell. This suggests that the promoter construct lacks some control elements. Since the GFP expression of the fusion protein is significantly lower than the expression of the promoter construct, it is possible that low levels of expression in tissues and cells expressing the plr-1::GFP transcriptional reporter remain undetectable.
• Primary Identifier: Expr12087

5 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
the feeding organ, a neuro-muscular pump in the head of the animal, used to ingest food, bacteria suspended in liquid, filter them out, grind them up and transport posteriorly into the instestine.	pharynx	esophagus	WBbt:0003681
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
posterior region, from rectum to the end	tail		WBbt:0005741
Neuron class of one ventral cord interneuron, few synapses.	AVG	lineage name: ABprpapppap	WBbt:0003850

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00023404	plr-1	Y47D3B.11	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018