WormMine: ExpressionPattern Expr12265

• Pattern: The localization of tetraThymosin-beta is shown in oocytes (in dissected gonads of adult worms), in embryos and in whole adult organisms. TetraThymosin-beta was maternally expressed and in the adult gonad diffusely present in the cytoplasm of the oocytes, with strong staining at the cell cortex where also actin is present. In the distal arm of the gonad, tetraThymosin-beta localized to the inside edges of the membrane cubicles surrounding germ cell nuclei. Here, tetraThymosin-beta did not localize to the lateral side of the membrane cubicles where actin is arranged in a 'honeycomb' structure (Strome, 1986). In two-cell stage embryos, tetraThymosin-beta was enriched in the cell-cell contact where actin was also concentrated. The tetraThymosin-beta signal was clearly apparent until the four-cell stage and became weaker later on. At the comma stage (290 min after the first cell division), tetraThymosin-beta staining was observed at the developing nerve ring that is the largest axonal bundle in the nematode body and that positions around the isthmus of the pharynx. TetraThymosin-beta was evident in this region even before a clear actin signal was apparent. In larvae and adults, immunofluorescence yielded a diffuse but specific staining of the entire worm body with enrichment at the intestinal tract and spermatheca. At these stages, in contrast to what is observed in developing embryo, no prominent signal was observed in the head region where the nerve ring is located. In young adults is expressed in theposterior bulb of the pharynx. These immunostaining patterns were reproducible in three or more experiments and in 20 or more worms or embryos.
• Primary Identifier: Expr12265

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
somatic cell that situates at the tip of a gonad arm.	distal tip cell	DTC	WBbt:0006865
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
the last, posterior bulb of the pharynx.	terminal bulb		WBbt:0003732

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006649	tth-1	F08F1.8	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	A developmental life stage of the nematode Caenorhabditis elegans that occurs from egg hatching until adulthood.		larva Ce	WBls:0000023
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 0-20min after first cleavage at 20 Centigrade. Contains 2 cells.		2-cell embryo Ce	WBls:0000007
	Also called zygote. -50min till 0min of first cleavage at 20 Centigrade. Contains 1 cell.		1-cell embryo Ce	WBls:0000006
	The C. elegans life stage spanning 20-40min after first cleavage at 20 Centigrade. Contains 4 cells.		4-cell embryo Ce	WBls:0000008