WormMine: AnatomyTerm WBbt:0003712

• Definition: Pharyngeal gland cell 1.
• Name: G1
• Primary Identifier: WBbt:0003712

4 Children

Definition	Name	Synonym	Primary Identifier
Pharyngeal gland cell 1P, before fusion into g1ARP.	g1P	lineage name: MSaaaaapap	WBbt:0004528
Pharyngeal gland cell 1AL.	g1AL	lineage name: MSaapaapaa	WBbt:0004532
Pharyngeal gland cell 1AR, before fusion into g1ARP.	g1AR	lineage name: MSpapaapaa	WBbt:0004530
Syncytial g1 gland cell, formed by fusion between g1AR and g1P cells.	g1ARP	g1AR	WBbt:0008350

0 Expression Clusters

7 Expression Patterns

• Primary Identifier: Expr643
• Remark: Reporter gene fusion type not specified. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).
• Pattern: wrt-3 is expressed from 3-fold stage onwards in the trinucleate pharyngeal gland cell g1. Variable expression is observed in seam cells and hypodermis, occasionally in single muscle cells and distal tip cells of older larvae and adults.

• Primary Identifier: Expr11043
• Pattern: F48A11.1 was expressed specifically in the period immediately preceding each moult. Expression appeared limited to the pharynx and specifically to the glandular cells g1 and g2 (5 nuclei) in the terminal bulb, the three m4 myo-epithelial cells (6 nuclei) in the metacorpus and the three m3 myo-epithelial cells (6 nuclei) in the pro- corpus. No GFP was detected elsewhere in transgenic nematodes despite careful examination of the reproductive tissues and intestine.

• Primary Identifier: Expr7
• Remark: Refer to Birchall,Fishpool & Albertson (1995) life_stage summary : postembryonic
• Pattern: Post-embryonic expression. L2-L4-- punctate signal in 6 cells in the body (coelomocytes), 2 ventral at level of Int3, 2 ventral anterior to developing gonad, 2 left and right subdorsal posterior to gonad. Weaker signal in muscle. Head-- 6 cells closely apposed to pharynx at the level of the nerve ring, 2 subdorsal, 4 ventral-subventral; single cell dorsal to second bulb of the pharynx. Pharynx-- g1 gland cell.

• Primary Identifier: Expr427
• Remark: Other strain-- UL882
• Pattern: Expression is seen in a number of components. Firstly, in embryos expression is seen from the ~20 cell stage until after elongation in most cells (excluding the E-cell lineage). Secondly, in larvae and adults expression is seen in the pharyngeal g1 gland cell. Finally, in larvae and adults two neuronal cell bodies located anteriorly to the terminal bulb show expression. This gene has similarity to human myelin transcription factor 1.

• Primary Identifier: Expr444
• Remark: In only 1 out of 4 strains did we observe expression.
• Pattern: Expression is seen in late larvae and adult stages in two components. In the pharynx the g1 gland cell shows expression, and within the body of the worm the spermathecal-uterine junction and uterine wall show expression.

• Primary Identifier: Expr1233
• Remark: GFP and antibody show the same expression pattern.
• Pattern: kel-1 protein became detectable at the 1.5-fold embryo, appearing as a dotted line at the midline of the head region. At the 2-fold embryo, and 3-fold embryo, observed more evidently as a central line along the lumen of the pharynx between posterior end of the buccal cavity and the terminal bulb. At embryonic stages up to the pretzel, but not larval stages, anti-KEL-1 stained a structure appearing to be perpendicular to the pharynx. With laser scanning confocal microscope, this structure appeared to be a ring surrounding the posterior metacorpus. Expression was observed along the pharyngeal lumen throughout the postembryonic development. Cells strongly express KEL-1 were the pharyngeal g1 gland cells.

• Primary Identifier: Expr179
• Remark: Other strain-- UL706
• Pattern: From early larval stages until adults, weak expression is seen in the g1 gland cell of the pharynx.

11 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes.		late cleavage stage embryo Ce	WBls:0000014

2 Parents

Definition	Name	Synonym	Primary Identifier
Secretory gland cell of the pharynx	Pharyngeal gland cell		WBbt:0005788
the last, posterior bulb of the pharynx.	terminal bulb		WBbt:0003732