WormMine: ExpressionPattern Expr862

• Pattern: At the L1 larval stage CEH-6ceh-6 is expressed in four pairs of bilaterally symmetric neurons in the lateral ring ganglion of the animal. These neurons are the RMDDLR, RMDVLR, AUALR and AVHLR neurons. The expression in RMDD and RMDV is weaker than in AUA and AVH. ceh-6 is also expressed in the excretory cell, very strongly with the lacZ reporter construct but more weakly with the antibody, which is probably due to the large volume of the nucleus. Despite the nuclear localization signal in the lacZ construct, the beta-galactosidase was occasionally expressed at such high levels that the excretory canals were also stained. Posterior to the excretory cell, the neurons SABVLR in the retro-vesicular ganglion express CEH-6ceh-6. Additional CEH-6-expressing cells in the body and tail were observed only with the antiserum, indicating that the ceh-6 reporters may not contain all promoter elements. In the body region, expression was observed in dividing P.na cells in the ventral nerve cord in L1 animals. The Pn.a expression is transient, appearing before the cell division of Pn.a and fading in the daughter cells. During the cell division, CEH-6 is localized to the cytoplasm of the dividing cells. It appears that the posterior daughters lose CEH-6 before that of the anterior daughter, as seen in the anterior Pn.ap cells. In the tail, CEH-6 expression in L1 animals is seen around the rectum in the five rectal cells B, Y, U, F and K. After K has divided, only the rectal cell K.a expresses CEH-6, though expression during the division was not monitored. At the L2 stage, a sixth cell becomes apparent that, based on its position at the very bottom of the rectum, is deduced to be P12.pa. Head and rectal expression of CEH-6 persists into adulthood. In addition, in adult animals four symmetric CEH-6 expressing cells are seen around the vulva, possibly one set of the vul cells, though did not determine their identity. During embryogenesis ceh-6 is expressed at the comma stage in two clusters one cluster corresponds to the ectodermal cells surrounding the anus (B, Y, U, F and K), and the other cluster corresponded to the cells in the head described above, many of which are located relatively close to each other at that stage in development.
• Primary Identifier: Expr862
• Remark: Reporter gene fusion type not specified.

33 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
posterior region, from rectum to the end	tail		WBbt:0005741
Four processes (canals) of the excretory canal cell each contain a central collecting lumen which feeds to a central lumenal canal in the cell body; the central canal forms a specialized membrane to release fluids into the excretory duct. These five canals form a continuous H-shaped channel which extends almost the full length of the body, generally in contact with the lateral hypodermis and the pseudocoelom.	excretory canal	canal	WBbt:0005775
anterior-most body region containing the pharynx.	head		WBbt:0005739
Rectal cell, postembryonic blast cell in male	B cell	lineage name: ABprppppapa	WBbt:0003825
Rectal cell, blast cell in male	F cell	lineage name: ABplppppapp	WBbt:0004799
descendent cell of P3, anterior	P3.a		WBbt:0006879
descendent cell of P2, anterior	P2.a		WBbt:0006878
descendent cell of P1, anterior	P1.a		WBbt:0006877
descendent cell of P7, anterior	P7.a		WBbt:0006883
descendent cell of P6, anterior	P6.a		WBbt:0006882
descendent cell of P5, anterior	P5.a		WBbt:0006881
descendent cell of P4, anterior	P4.a		WBbt:0006880
descendent cell of P11, anterior	P11.a		WBbt:0006887
descendent cell of P10, anterior	P10.a		WBbt:0006886
descendent cell of P9, anterior	P9.a		WBbt:0006885
descendent cell of P8, anterior	P8.a		WBbt:0006884
descendent cell of P12, anterior	P12.a		WBbt:0006888
Rectal cell, postembryonic blast cell	K cell	lineage name: ABplpapppaa	WBbt:0004499
Rectal cell, postembryonic blast cell in male	U cell	lineage name: ABplppppapa	WBbt:0004942
Ring motor neuron/interneuron, many synapses	RMDVL	lineage name: ABalppapaaa	WBbt:0005031
Neuron, process runs with amphid processes but lacks cillialted ending	AUAR	lineage name: ABpraaappppp	WBbt:0003871
Neuron, process runs with amphid processes but lacks cillialted ending	AUAL	lineage name: ABalpppppppp	WBbt:0003872
Neuron, mainly postsynaptic in ventral cord and presynaptic in the ring	AVHR	lineage name: ABalappapaa	WBbt:0003848
Neuron, mainly postsynaptic in ventral cord and presynaptic in the ring	AVHL	lineage name: ABalapaaaaa	WBbt:0003849
Ring motor neuron/interneuron, many synapses	RMDVR	lineage name: ABarapppaaa	WBbt:0005029
Ring motor neuron/interneuron, many synapses	RMDDR	lineage name: ABarappapaa	WBbt:0005038
Ring motor neuron/interneuron, many synapses	RMDDL	lineage name: ABalpapapaa	WBbt:0005039
Ring interneuron, anteriorly projecting process that runs sublaterally, synapses to anterior body muscles in L1	SABVL	lineage name: ABplppapaaaa	WBbt:0004998

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000431	ceh-6	K02B12.1	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024