WormMine: ExpressionPattern Expr842

• Pattern: GFP expression was observed in about 30 neurons of adult hermaphrodite worms. pida-1::GFP 7.6 expression was prominent in a subset of neurons in the head nerve ring, around the vulva, in a few cells in the ventral cord, and in the tail. Although the GFP was concentrated in the nucleus, the whole cell body including axons was labelled. Labeled processes included two lateral and one dorsal axon and a bundle of ventral axons running the length of the body, a pair of lateral axon bundles running from the head to the tip of the nose, and a pair of axons running from the anal region to the tip of the tail. GFP expression was observed as early as in late embryos. Larvae showed expression in the head and the tail, whereas GFP expression near the vulva and in the ventral cord was only seen in late L4 and adult animals. The expression pattern in dauer larvae was similar to that in other larval stages. Males exhibited many more GFP-expressing cells in the tail and additional cells in the head, and expression in the ventral cord differed from that observed in hermaphrodites. The GFP-expressing neurons in the hermaphrodite head were identified as ADE, ALA, ASI, ASK, AUA, ASG, AVH, and AVJ. Expression in ASG, AVH, and AVJ was not as bright as in other cells and was variable from individual to individual. A few other GFP-expressing anterior neurons could not be identified unequivocally. The fluorescent cells in the midsection of the animal were identified as the vulval motoneurons VC and HSN and the vulval uv1 cells. These are all hermaphrodite-specific and GFP expression was not observed until the late L4 larval stage. A lateral pair of neurons in the posterior half of the animal with weak GFP expression was identified as PDE. They possess ventrally directed processes entering the ventral nerve cord. In the preanal ganglion a pair of neurons with weak GFP expression was identified as PVP. Three pairs of GFP-expressing cells in the tail were identified as PHA, PHB, and PHC. Corresponding cells for the dorsal axon and a preanal structure have not been identified. The latter short process originates from the ventral nerve cord and peters out and terminates at the hypodermis.
• Primary Identifier: Expr842
• Remark: Reporter gene fusion type not specified. cgc6469 mentioned that this reporter gene is transcriptional fusion.
• Subcellular Localization: GFP was concentrated in the nucleus, the whole cell body including axons was also labelled.

30 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Neuron class of six motoneurons in the ventral cord, which innervate ventral body muscles and the vm2 muscles of the vulva.	VC neuron		WBbt:0005304
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNL	lineage name: ABplapppappa	WBbt:0004758
Herm. specific motor neurons (die in male embryo), innervate vulval muscles, serotonergic	HSNR	lineage name: ABprapppappa	WBbt:0004757
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASGL	lineage name: ABplaapapap	WBbt:0003892
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASGR	lineage name: ABpraapapap	WBbt:0003891
Neuron, dopaminergic of postderid sensillum	PDEL	lineage name: V5L.paaa	WBbt:0004383
Neuron, dopaminergic of postderid sensillum	PDER	lineage name: V5R.paaa	WBbt:0004382
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
one member of amphid neurons, single ciliated ending, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASIL	lineage name: ABplaapapppa	WBbt:0003888
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASIR	lineage name: ABpraapapppa	WBbt:0003887
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASKR	lineage name: ABpraaaapppa	WBbt:0003883
one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil	ASKL	lineage name: ABalpppapppa	WBbt:0003884
Phasmid neurons, probably chemosensory	PHAL	lineage name: ABplpppaapp	WBbt:0004366
Phasmid neurons, probably chemosensory	PHAR	lineage name: ABprpppaapp	WBbt:0004365
Neuron class of one neuron, sends processes laterally and along dorsal cord.	ALA	lineage name: ABalapppaaa	WBbt:0003955
Phasmid neuron, probably chemosensory	PHBR	lineage name: ABprapppappp	WBbt:0005659
Phasmid neurons, probably chemosensory	PHBL	lineage name: ABplapppappp	WBbt:0004364
uterine-vulval cell proximal to the vulva.	uv1		WBbt:0006791
Anterior deirid, sensory neuron, dopaminergic	ADER	lineage name: ABprapaaaapa	WBbt:0004007
Anterior deirid, sensory neuron, dopaminergic	ADEL	lineage name: ABplapaaaapa	WBbt:0004009
Interneuron, cell body in lumbar ganglion, projects along ventral cord to nerve ring	PVPL	lineage name: ABplppppaaa	WBbt:0004082
Interneuron, cell body in lumbar ganglion, projects along ventral cord to nerve ring	PVPR	lineage name: ABprppppaaa	WBbt:0004078
Neuron, striated rootlet in male, possibly sensory in tail spike	PHCL	lineage name: TL.pppaa	WBbt:0004362
Neuron, striated rootlet in male, possibly sensory in tail spike	PHCR	lineage name: TR.pppaa	WBbt:0004118
Neuron, process runs with amphid processes but lacks cillialted ending	AUAR	lineage name: ABpraaappppp	WBbt:0003871
Neuron, process runs with amphid processes but lacks cillialted ending	AUAL	lineage name: ABalpppppppp	WBbt:0003872
Neuron, synapses like AVHL/R	AVJR	lineage name: ABalapppppa	WBbt:0003846
Neuron, mainly postsynaptic in ventral cord and presynaptic in the ring	AVHR	lineage name: ABalappapaa	WBbt:0003848
Neuron, synapses like AVHL/R	AVJL	lineage name: ABalapapppa	WBbt:0003847
Neuron, mainly postsynaptic in ventral cord and presynaptic in the ring	AVHL	lineage name: ABalapaaaaa	WBbt:0003849

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002048	ida-1	B0244.2	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	All C. elegans development stages, including embryo, larva and adult stage.		all stages Ce	WBls:0000002