WormMine: ExpressionPattern Expr2572

• Pattern: GFP expression was first observed in several posterior cells of comma stage embryos, then in the excretory cell and some head neurons in threefold stage embryos. In L1 to adult worms, continued expression of GFP was observed in several head neurons, the excretory cell and the excretory gland cells, as well as the sphincter muscles. One of the head neurons was identified as the AVG neuron based on the cell position and morphology. In addition, in L4 worms, GFP was seen in a set of hypodermal cells surrounding the vulva, which persisted in the hypodermal cells flanking the vulval opening in adult worms. No GFP expression was detected in the sensory neurons. Faint GFP expression was infrequently detected in one to four neurons in the ventral cord. No expression was observed in the sex myoblasts, nor was GFP detected in the body wall muscles at any stages.
• Primary Identifier: Expr2572
• Reporter Gene: [unc-71::gfp] transcriptional fusion. To generate the unc-71 transcriptional GFP construct pCZ411, the 1.7 kb upstream regulatory region of unc-71 was amplified by PCR, and inserted into pPD95.79.

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with cell body associated with the ventral nerve cord.	ventral cord neuron	ventral cord motoneuron	WBbt:0005300
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
gland cell of the secretory-excretory system, sends processes to ring, opens into excretory duct.	excretory gland cell	exc gl	WBbt:0005776
a saddle-shaped muscle cell that encircles the intestinal-rectal valve	anal sphincter muscle	lineage name: ABprpppppap	WBbt:0005798
H-shaped cell associated with the excretory system, largest cell in C. elegans.	excretory cell	excretory canal cell	WBbt:0005812
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
entire syncytium of hyp7	hyp7 syncytium	hyp7	WBbt:0005734
Neuron class of one ventral cord interneuron, few synapses.	AVG	lineage name: ABprpapppap	WBbt:0003850

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006804	unc-71	Y37D8A.13	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020