WormMine: ExpressionPattern Expr13980

• Pattern: In males, DDO-3::mCherry was localized in the excretory pore cell, head tip cells, hypodermal cells, seminal vesicle cells, tail cells, and spicule cells. By contrast, DDO-3::mCherry was also localized in the tail fan from the adult stage, suggesting that DDO-3 is secreted in a SP-dependent manner from the cells where it is produced and transferred to the tail fan. Interestingly, DDO-3::mCherry was localized in vesicle-like structures in the seminal vesicle, where spermatids are stored until ejaculation. Because the seminal vesicle is composed of 20 secretory cells, we presumed that DDO-3 was secreted from the seminal vesicle into the seminal fluid and transferred to the hermaphrodite during mating. To test this possibility, we examined the uteri of hermaphrodites just after mating with transgenic males carrying the ddo-3::mCherry reporter gene. DDO-3::mCherry was dispersed throughout the uterus, suggesting that DDO-3 is a seminal fluid protein. Taken together, these results are consistent with the idea that DDO-3 is secreted from the seminal vesicle into the seminal fluid in a SP-dependent manner, and then transferred to the hermaphrodite uterus through mating. This fusion protein was also present in coelomocytes of males. During larval stages L1 and L2, localization of DDO-3::mCherry outside coelomocytes was limited to the excretory pore cell, implying that DDO-3 expressed in excretory pore cells is secreted into the body fluid. Indeed, at least in the adult stage, DDO-3::mCherry in excretory pore cells was localized to vesicle-like structures.
• Primary Identifier: Expr13980

8 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
A free-floating spherical cell lying in the pseudocoelomic cavity of larvae and adult C. elegans which can endocytose many compounds, possibly for immune surveillance. There are six coelomocytes in adult hermaphrodites, and they display prominent cytoplasmic inclusions and vacuoles.	coelomocyte		WBbt:0005751
posterior region, from rectum to the end	tail		WBbt:0005741
	copulatory spicule		WBbt:0005312
anterior-most body region containing the pharynx.	head		WBbt:0005739
The outlet of the excretory sinus to the exterior environment through the cuticle, on the ventral side of the head, just behind the nerve ring.	excretory pore	pore	WBbt:0005778
Gonochoristic organism that can produce male gametes.	male	male organism	WBbt:0007850
part of male gonad that holds mature sperm, made of a total of 12 nuclei.	seminal vesicle	gon_male_sves	WBbt:0006870

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00017648	ddo-3	F20H11.5	Caenorhabditis elegans

2 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024