WormMine: ExpressionPattern Expr2563

• Pattern: The expression of ubc-25::gfp begins in gastrulation stage embryos shortly before morphogenesis starts. UBC-25::GFP fluorescence could be identified in precursor cells of neurons and muscle cells, but also in other cell types, like hypodermal precursor cells. After hatching of the L1 larva and in all subsequent life stages, ubc-25::gfp is expressed strongest in the pharyngeal muscle and in the anal muscle cells. In L4 stage animals and adolescent hermaphrodites ubc-25::gfp is also expressed in the vulval muscles. Furthermore, UBC-25::GFP fluorescence was identified in all four nerve chords and in neurons. A weaker level of UBC-25::GFP is detected in all body wall muscles. No post-embryonic ubc-25::gfp expression was observed in any non-neuromuscular tissue or cell type.
• Primary Identifier: Expr2563
• Subcellular Localization: UBC-25::GFP is located in the cytosol of the corresponding cells and is typically slightly enriched in the nuclei.

7 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
type of cells that make up muscle layers in the pharynx.	pharyngeal muscle cell		WBbt:0005451
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
a single, H-shaped cell which lies just above the anus and connects the roof of the anal canal to the dorsal bodywall; its contractions act to increase the size of the anal opening by lifting the roof of the rectum and hence facilitate expulsion of intestinal contents.	anal depressor muscle	dilator muscle	WBbt:0004292
a bundle of nerve processes that runs along the dorsal mid-line of the animal.	dorsal nerve cord	dorsal cord	WBbt:0006750
nerve cord positioned at the lateral flanks of the animal, consists of processes of the neuron classes BDU, CAN, PVD and ALA.	lateral nerve cord		WBbt:0006769

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006720	ubc-25	F25H2.8	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003