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Expr9916
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RAB-6.2::GFP is highly expressed in body wall muscles, pharyngeal and vulval muscles, hypodermis, intestine, the gonad, coelomocytes, seam cells, vulval epithelia, distal tip cells, ventral cord dendrites, and neurons. We also introduced this transgene into nematodes that also express a Pglr-1::monomeric RFP (mRFP) transgene (Shim et al., 2004), and we found that RAB-6.2::GFP is expressed in the GLR-1-expressing command interneurons. |
RAB-6.2 is localized to punctate structures in the neuron cell body and along the ventral cord dendrites. We introduced the Pglr-1::cerulean:: rab-6.2 transgene into nematodes that express the Golgi resident protein mannosidase (MANS)::YFP (Rolls et al., 2002; Glodowski et al., 2005) and observed nearly complete colocalization between Cerulean::RAB-6.2 and MANS::YFP, indicating that RAB-6.2 is localized on or near Golgi structures. |
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Expr10580
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The sol-2 promoter drives expression of GFP in many head and tail neurons, including neurons that express the GLR-1 subunit, as well as the SOL-1 auxiliary subunit (Brockie et al., 2001a; Zheng et al., 2004). SOL-2 is also expressed in neurons that do not express either GLR-1 or SOL-1. SOL-2 is expressed in the command interneurons, as shown by coexpression of mCherry driven by the nmr-1 promoter. |
SOL-2 colocalizes and is associated with SOL-1 and GLR-1 at synaptic sites. |
