WormMine: ExpressionPattern Expr12844

• Pattern: Specific UNC-34 staining was detected with anti-EVH2 antibodies in embryos at the beginning of ventral enclosure. UNC-34 specifically localized to apical junctions of these migrating epidermal cells as previously described using an UNC-34::GFP reporter (Sheffield et al., 2007). Later in embryogenesis, UNC-34 was also found in a pattern that suggested it localizes to junctions of the epithelial cells that line the inside of the pharynx. UNC-34 localization to these structures decreased after the first larval stage, but was observed strongly at apical junctions of the developing vulva. Anti-EVH2 antibodies revealed broad expression of UNC-34 in the nervous system that was missing from the mutants unc-34(e951) and unc-34(gm104). UNC-34 localized to axons with fainter cytoplasmic staining in neuronal cell bodies. The nerve ring, the major C. elegans neuropil that contains the highest concentration of axons, displayed the most intense staining, while the dorsal nerve cord (DNC) and ventral nerve cord (VNC) also expressed detectable UNC-34. Nerve ring staining was detected as early as the two-fold stage of embryogenesis and the intensity of axonal staining increased until late embryogenesis. UNC-34 axon expression continued into adulthood although it became fainter in older larvae and adults.
• Primary Identifier: Expr12844

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Complement of nervous tissue (neurones, nerves, receptors and support cells) serving to detect, relay and coordinate information about an animal`s internal and external environments and to initiate and integrate its effector responses and activities.	nervous system		WBbt:0005735
cell of epithelium, the layer of cells that cover the external surface of an organism as well as forming an inner lining between the tissues and organs of that organism and the external environment. In C. elegans epithelial cells include epithelium of the alimentary tract, intestinal cells, hypodermis, interfacial cells and somatic gonad cells.	epithelial cell	epithelium	WBbt:0003672

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006770	unc-34	Y50D4C.1	Caenorhabditis elegans

0 Life Stages