WormMine: ExpressionPattern Expr9638

• Pattern: nhr-67::gfp was expressed at all postembryonic stages in neurons of the head. nhr-67::gfp expression was not observed in uterine or vulval precursor in embryos or L1 larvae. In the L2 stage, nhr-67::gfp expression was observed in the 4 pre-VU precursor cells (Z1.ppa, Z1.ppp, Z4.aaa, Z4.aap) that become the AC and the three VU cells that give rise to the ventral uterus. Expression of nhr-67::gfp in the 4 pre-VU precursor cells appeared at or shortly after their birth. In the mid- to late-L2 stage, expression of nhr-67::gfp in the three VU cells decreased, while remaining at high levels (or possibly increasing) in the AC. During the L3 and early L4 stages, consistent and continual bright nhr-67::gfp expression was observed in the AC and more variable weak levels of expression were found in the six adjacent pi cells. nhr-67::gfp expression was very rarely detected in all 12 pi cells after their dorsal-ventral division. From early L4 through adulthood, weak expression of nhr-67::gfp was observed in all 8 UTSE cells or syncytium, suggesting that expression is turned off in all pi cells after their division and then re-expressed later in UTSE. Expression of nhr-67::gfp was observed in the UTSE before AC fusion. Bright expression of nhr-67::gfp in the AC nucleus was not observed after mid-L4, indicating that it is reduced after fusion of the AC with the UTSE syncytium.
• Primary Identifier: Expr9638

9 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522
membranous cell attaches the uterus to the lateral epidermis (seam) and forms a thin laminar process dorsal to the vulva. formed by fusion of eight pi cell progeny and AC.	uterine seam cell	utse	WBbt:0006789
Ventral Uterine precursor cell.	ventral uterine precursor	VU	WBbt:0006780
post-embryonic cell of pedigree Z4.aaa	Z4.aaa		WBbt:0007688
post-embryonic cell of pedigree Z1.ppp	Z1.ppp		WBbt:0007667
any of six ventral uterine intermediate precursor cells that are adjacent to the anchor cell and whose progeny are fated to become uv1 and utse cells.	uterine pi cell		WBbt:0007813
post-embryonic cell of pedigree Z1.ppa	Z1.ppa		WBbt:0007628
post-embryonic cell of pedigree Z4.aap	Z4.aap	VU	WBbt:0007707

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003657	nhr-67	C08F8.8	Caenorhabditis elegans

4 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024