WormMine: ExpressionPattern Expr544

• Pattern: LIN-12::GFP protein is initially detectable in both Z1.ppp and Z4.aaa, and later in development becomes detectable in the presumptive VU but not in the presumptive AC. The LIN-12:: GFP protein is initially detectable in all six VPCs. However, in the mid-L3 stage, at the time of VPC specification, LIN-12::GFP is reduced in P6.p relative to the other VPCs, notably with respect to P5.p and P7.p. High LIN-12::GFP accumulation is always seen in the daughters of P5.p and P7.p, but LIN-12::GFP accumulation is not seen in the daughters of P6.p. There is variable accumulation of LIN-12::GFP in the daughters of P3.p, P4.p and P8.p.
• Primary Identifier: Expr544
• Remark: Starting plasmid pLB30 fully rescues lin-12(null) mutant. Integrated strain arIs41[lin-12::GFP] yields mostly wild-type hermaphrodites, with <5% showing weak lin-12 gain-of-function phenotype.
• Subcellular Localization: In Z1.ppp and Z4.aaa, and in other gonadal cells, LIN-12::GFP is detectable over the entire surface of cells. However, in the VPCs, LIN-12::GFP is visible in the perinuclear region, which is probably endoplasmic reticulum/Golgi, and at the ventral (apical) surface and at the junctions between VPCs.

17 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
	P6.p hermaphrodite		WBbt:0008125
descendent cell of post-embryonic blast cell P5, posterior, in hermaphrodite.	P5.p hermaphrodite		WBbt:0008121
descendent cell of post-embryonic blast cell P4, posterior, in hermaphrodite.	P4.p hermaphrodite		WBbt:0008117
descendent cell of post-embryonic blast cell P3, posterior in hermaphrodite.	P3.p hermaphrodite		WBbt:0008112
	P8.p hermaphrodite		WBbt:0008133
	P7.p hermaphrodite		WBbt:0008129
cell, posterior descendent of P7.p	P7.pp		WBbt:0006984
cell, anterior descendent of P7.p	P7.pa		WBbt:0006983
post-embryonic cell of pedigree P5.pa	P5.pa		WBbt:0007252
post-embryonic cell of pedigree P5.pp	P5.pp		WBbt:0007255
Anterior daughter cell of P3.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite.	P3.pa	lineage name: P3.pa	WBbt:0004155
Posterior daughter cell of P3.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite.	P3.pp	lineage name: P3.pp	WBbt:0004153
Anterior daughter cell of P4.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite.	P4.pa	lineage name: P4.pa	WBbt:0004151
Posterior daughter cell of P4.p, fuses into postembryonic large hypodermal syncytium in hermaphrodite.	P4.pp	lineage name: P4.pp	WBbt:0004149
post-embryonic cell of pedigree Z4.aaa	Z4.aaa		WBbt:0007688
post-embryonic cell of pedigree Z1.ppp	Z1.ppp		WBbt:0007667

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003001	lin-12	R107.8	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035